诊断学理论与实践2012，Vol.11Issue(5)：459-465,7.DOI:10.3969/j.issn.1671-2870.2012.05.007
胰腺实性假乳头肿瘤诊断及鉴别诊断标志物的筛选
Immunohistochemical markers for the differential diagnosis of pancreatic solid-pseudopapillary neoplasm
郭滟 1费晓春 1谢静 1吴华成 1金晓龙 1肖家诚1
作者信息
- 1. 上海交通大学医学院附属瑞金医院病理科,上海 200025
- 折叠
摘要
Abstract
Objective To study the diagnostic value of various immunohisochemical markers and to identify the most useful marker for the definitive diagnosis of pancreatic solid-pseudopapillary neoplasm (SPN). Methods: Immunohistochemical staining CD99, E-cadherin, β-catenin, PI20 and other markers used for the diagnosis of SPN were tested in tissue microarray blocks from 55 cases of SPN, 51 cases of pancreatic neuroendocrine tumor (β-NET), and 54 cases of pancreatic adenocarcinoma (PAC). Surgical specimens from 19 SPN,6 pancreatic acinar cell carcinoma (ACC) and 1 pan-creatoblastoma (PB) were also investigated. Results: All the SPN cells exhibited paranuclear dot-like immunoreactivity of CD99 regardless the clinicopathological or morphological features. Forty of the 51 P-NET were positive for CD99 on cell membrane and/or cytoplasm. Four of the 54 PAC and 1 PB showed faint membrane staining. None of the ACC was reactive for CD99. All the SPN cases were negative for E-cadherin and positive for nuclear and cytoplasm β-catenin. Forty-six P-NET, 52 PAC, 6 ACC and 1 PB were E-cadherin membrane positive. All the P-NET, PAC, ACC, PB cases were β-catenin membrane positive. Fifty-nine SPN and 38 P-NET were PI20 cytoplasm positive. Six SPN, 8 P-NET, 48 PAC, 5 ACC and 1 PB were PI 20 membrane positive. Seventy-one SPN, 19 P-NET and 4 PAC were PR nuclear positive. Fifty-nine SPN, 46 P-NET and 5 PAC were positive for CD10. Sixty-six SPN, 45 P-NET and 2 PAC were positive for CD56. Seventy-two SPN, 48 P-NET and 3 PAC were positive for NSE. All the ACC and PB were negative for PR, CD 10, CD56 and NSE. Conclusions: The unique dot-like staining pattern of CD99 in SPN is the most useful immuno-marker for definitive diagnosis of SPN and differentiation from other pancreatic tumors. β-catenin and E-cadherin are also helpful for the diagnosis of SPN.关键词
CD99/β-catenin/E-cadherin/组织芯片/免疫组织化学Key words
CD99/β-catenin/E-cadherin/Tissue microarray /Immunohistochemistry分类
医药卫生引用本文复制引用
郭滟,费晓春,谢静,吴华成,金晓龙,肖家诚..胰腺实性假乳头肿瘤诊断及鉴别诊断标志物的筛选[J].诊断学理论与实践,2012,11(5):459-465,7.
