诊断学理论与实践2012，Vol.11Issue(5)：502-506,5.DOI:10.3969/j.issn.1671-2870.2012.05.016
中药提取物诱导血液肿瘤细胞凋亡的初步研究
Study on CHP-induced cell apoptosis in hematopoietic malignancies
摘要
Abstract
Objective: To study the effect of CHP (a Chinese traditional medicine extract) on apoptosis of acute myeloid leukemia cell line Kasumi-1 and follicular lymphoma cell line Su-DHL-4. Methods: Kasumi-1 cells and Su-DHL-4 cells were treated respectively with CHP at the concentration of 50 μg/mL. The effect of CHP was evaluated by cell growth, cell morphology and markers of cell apoptosis. The plasma membrane translocation of phosphatidylserine, the mitochondrial transmembrane potential, and the expression of anti-apoptotic protein Bcl-2 were detected by flow cytometry. Results: CHP inhibited significantly the cell proliferation of Kasumi-1 cells and Su-DHL-4 cells. After 48 h treatment with CHP, the rates of growth inhibition of Kasumi-1 and Su-DHL-4 cells were (69.45 ±7.21)% and (82.44 ±5.86)% , respectively, and typical apoptotic cells could be observed. In addition, compared with the two control groups, the percentage of Annexin V positive cell in these two cell lines treated with CHP for 24h were raised significantly from (10.58+1.22)% and (4.30+0.52)% to ( 53.53.±6.07)% and (59.87±5.56)%, respectively (P<0.01). Meanwhile, the mitochondrial transmembrane potential of these two cell lines increased markedly from (6.67±0.64)% and (7.20±2.33)% to (31.13±1.80)% and (55.97±7.33)% (P<0.01). In addition, the expression of Bcl-2 in Su-DHL-4 cells was obviously down-regulated. Conclusions: The traditional Chinese medicine extract CHP could induce apoptosis in both Kasumi-1 cells and Su-DHL-4 cells.关键词
细胞凋亡/中药提取物/流式细胞术Key words
Cell apoptosis/ Chinese herbal preparation/ Flow cytometry分类
医药卫生引用本文复制引用
夏迪,贾培敏,马瑜珊,童建华..中药提取物诱导血液肿瘤细胞凋亡的初步研究[J].诊断学理论与实践,2012,11(5):502-506,5.基金项目
国家自然科学基金(30971275和81170508) （30971275和81170508）
上海市领军人才"国家队"专项资金 （）
上海市科委优秀学术带头人计划项目(11XD1403500) （11XD1403500）
