中国病理生理杂志2012，Vol.28Issue(11)：1928-1932,5.DOI:10.3969/j.issn.1000-4718.2012.11.002
MicroRNA-29a对大鼠心肌细胞Bcl-2和Mcl-1表达的调控作用及其机制
Roles of microRNA-29a in expression of Bcl-2 and Mcl-1 in rat cardiomyocytes
摘要
Abstract
AIM; To investigate the regulatory mechanisms of microRNA -29a (miR -29a) on the expression of Bcl - 2 and Mcl - 1 in rat cardiomyocytes ( CM cells) . METHODS: The CM cells were isolated from the hearts of new-born rats and transfected with miR - 29a mimic ( 100 nmol/L) by Lipofectamine RNAiMAX. The expression of Bcl - 2 and Mcl - 1 at mRNA and protein levels was detected by real - time fluorescence quantitative PCR and Western blotting. The luciferase assay was performed in HEK293T cells and CM cells, which were co - transfected with plasmid DNA and miRNA using Lipofectamine 2000. RESULTS: Transfection of miR - 29a mimics significantly reduced the expression levels of Bcl -2 and Mcl - 1 in CM cells as compared with the control cells (P <0. 05). In addition, HEK293T cells co -transfected with miR - 29a mimic and Bcl - 2 - 3 ' UTR - WT or Mcl - 1 - 3 ' UTR - WT plasmid significantly reduced the luciferase activity as compared with control group (P <0.05). While CM cells transfected with miR -29a inhibitor and Bcl -2-3' UTR - WT or Mcl - 1 - 3 ' UTR - WT plasmid in succession, the luciferase activity was increased inversely ( P < 0. 05 ) . CONCLUSION: miR - 29a may regulate apoptosis by targeting the bcl - 2 and mcl -1 genes.关键词
MicroRNA-29a/细胞凋亡/B细胞白血病/淋巴瘤-2/髓样细胞白血病-1Key words
MicroRNA-29a/Apoptosis/B-cell leukemia/lymphoma-2/Myeloid cell leukemia-1分类
医药卫生引用本文复制引用
张振辉,尤祥宇,刘少军,刘连,刘世明..MicroRNA-29a对大鼠心肌细胞Bcl-2和Mcl-1表达的调控作用及其机制[J].中国病理生理杂志,2012,28(11):1928-1932,5.基金项目
广东省自然科学基金资助项目(No.9151052002000006) （No.9151052002000006）
国家自然科学基金青年项目(No.81201453) （No.81201453）
广州市医药卫生科技项目(No.201102A213020) （No.201102A213020）
