中国病理生理杂志2012，Vol.28Issue(11)：1938-1942,5.DOI:10.3969/j.issn.1000-4718.2012.11.004
巨噬细胞移动抑制因子经Rho激酶途径促进人胚肺成纤维细胞表型转化
Macrophage migration-inhibitory factor induces myofibroblastic phenotype transformation of human embryonic lung fibroblasts via Rho-kinase in vitro
摘要
Abstract
AIM: To investigate the influence and the mechanism of recombinant macrophage migration - in-hibitory factor (rMIF) on fibroblasts. METHODS: Cultured human embryonic lung fibroblasts ( MRC -5 cells) were di-vided into 2 groups: the cells in treatment group were treated with rMIF (25～100 μg/L) for 24 h or 48 h, and the control cells were without rMIF treatment. The mRNA expression of a - SMA was examined by RT - PCR. The protein level of a - SMA induced by rMIF was quantified by Western blotting. Half an hour before 100 μg/L rMIF challenge, Y27632 was added to the cells of 2 groups. After challenged for 48 h, the total RNA and protein were extracted, and the expression of a - SMA at mRNA and protein levels was determined by means of RT - PCR and Western blotting. After challenged by 100 μg/L rMIF for 6 h, 12 h, 24 h and 48 h, the cell total protein was extracted, and the protein level of a - SMA induced by rMIF was quantified by Western blotting. RESULTS: After stimulation for 24 h, rMIF did not increase the a - SMA mR-NA synthesis compared with control. After stimulation for 48 h, rMIF significantly increased the expression of a - SMA mRNA and protein in a dose - dependent manner compared with control ( r = 0. 697 and r = 0. 957, both P < 0. 01 ) . Y27632 pretreatment prevented the increase ( P < 0. 01) . The amount of phosphorylated MYPT1 increased at 6 h ( P < 0. 01) , reached the maximum at 12 h ( P < 0. 01) , and decreased but still higher than the control at 24 h and 48 h ( P < 0. 01). CONCLUSION: rMIF increases the α - SMA synthesis in MRC -5 cells and Rho - kinase regulates this process, suggesting that MIF may play important roles in the pathogenesis of airway remodeling.关键词
巨噬细胞移动抑制因子/成纤维细胞/肌成纤维细胞/α-平滑肌肌动蛋白/Rho相关激酶类Key words
Macrophage migration-inhibitory factor/Fibroblasts/Myofibroblasts/α-Smooth muscle actin/Rho-associated kinases分类
医药卫生引用本文复制引用
陈培芬,邱智辉,张香梅,黎振兴,罗雅玲,赖文岩,孙秀才..巨噬细胞移动抑制因子经Rho激酶途径促进人胚肺成纤维细胞表型转化[J].中国病理生理杂志,2012,28(11):1938-1942,5.基金项目
国家自然科学基金资助项目(No.30770936) （No.30770936）
