中国病理生理杂志2012，Vol.28Issue(11)：1982-1985,4.DOI:10.3969/j.issn.1000-4718.2012.11.013
原发性肾病综合征并发急性肾损伤患者血清及肾组织中NGAL的表达及意义
Expression of NGAL in serum and renal tissues of patients with primary nephrotic syndrome and acute kidney injury
刘静 1李绍梅 1薛雯 1温文龙 1杨林 1杨万霞 1王建荣1
作者信息
- 1. 河北医科大学第二医院肾内科,河北,石家庄,050000
- 折叠
摘要
Abstract
AIM; To observe the changes of neutrophil gelatinase -associated lipocalin (NGAL) level in ser-um and renal tissues of the patients with primary nephrotic syndrome (PNS) and acute kidney injury (AKI). METH-ODS : Seventy - two PNS patients were selected in the study and divided into 2 groups according to the pathological results of renal biopsy. The patients in PNS + AKI group included 15 cases of PNS with acute tubular necrosis ( ATN) , in which there were 10 cases of minimal change disease ( MCD) with ATN and 5 cases of mesangial proliferative glomerulonephritis ( MsPGN) with ATN. The patients in PNS alone group included 57 cases of PNS without ATN. According to the pathologi-cal types, they were divided into MCD group (24 cases) , membranous nephropathy (MN) group (23 cases) and MsPGN group (10 cases). Serum samples from 15 healthy persons and 5 cases of normal renal tissues were used as controls. The serum levels of NGAL were detected by ELISA. The distribution and expression of NGAL in the renal tissues were observed by immunohistochemical method. RESULTS: ( 1) The serum level of NGAL in PNS alone group (including MCD group, MN group and MsPGN group) was significantly higher than that in normal control group. Compared with MCD group and MsPGN group, the serum level of NGAL in MN group increased significantly. The renal tissue positive expression index of NGAL in MCD group, MN group and MsPGN group was significantly higher than that in normal control group. (2) The se-rum level of NGAL and the renal tissue positive expression index of NGAL in MCD + ATN group and MsPGN + ATN group were significantly increased compared with PNS alone group. ( 3 ) The serum level of NGAL was positively correlated with 24 h urine protein, blood urea nitrogen and serum creatinine. No relation with serum albumin and β2 microglobulin was ob-
served. The serum level of NGAL was positively correlated with the NGAL level in the renal tissue. CONCLUSION: The serum level of NGAL can be used as one of the sensitive indexes for early and non - invasive detection of PNS with AKI.
关键词
原发性肾病综合征/中性粒细胞明胶酶相关脂质运载蛋白/急性肾损伤Key words
Primary nephrotic syndrome/Neutrophil gelatinase -associated lipocalin/Acute kidney injury分类
医药卫生引用本文复制引用
刘静,李绍梅,薛雯,温文龙,杨林,杨万霞,王建荣..原发性肾病综合征并发急性肾损伤患者血清及肾组织中NGAL的表达及意义[J].中国病理生理杂志,2012,28(11):1982-1985,4.
