中国当代儿科杂志2012，Vol.14Issue(9)：708-712,5.
miRNA-126/miRNA-126*在大鼠正常胎肺发育过程中的作用
Role of miRNA-126/miRNA-126* in the fetal lung development of rats
摘要
Abstract
Objective To investigate the expression and role of miRNA-126/miRNA-126* in the fetal lung development of rats. Methods Twelve pregnant Sprague-Dawley rats were randomly divided into 3 groups and the fetal rats were removed at 16, 19 and 21 days of gestation respectively. Hematoxylin and eosin staining was performed to observe lung morphology of fetal rats. Then microRNA (miRNA) microarray was used to study the expression patterns of raiRNA-126/miRNA-126* in fetal lungs at the three time points. And miRNA-126* was selected for further study by real-time PCR. Results There was no evident difference in the expression of miRNA-126 among the three groups, however the expression level of miRNA-126* increased gradually as the fetal lung developed. The real-time PCR result further showed that expression of miRNA-126* increased gradually with lung development, displaying significant differences among the three groups ( P < 0. 05 ). Conclusions miRNA-126 * may play an important role in development of the fetal lung in rats.关键词
肺发育/miRNA-126*/miRNA-126/大鼠Key words
Lung development/miRNA-126*/miRNA-126/Rats分类
医药卫生引用本文复制引用
杨洋,阚清,邱洁,浦晓丹,张盼,张晓群,周晓玉..miRNA-126/miRNA-126*在大鼠正常胎肺发育过程中的作用[J].中国当代儿科杂志,2012,14(9):708-712,5.基金项目
江苏省卫生厅面上项目(No.H200642). （No.H200642）
