中国骨伤2012，Vol.25Issue(10)：846-851,6.DOI:10.3969/j.issn.1003-0034.2012.10.015
膝关节骨关节炎发病过程中HSP70对软骨细胞凋亡的影响
Effect of HSP70 on apoptotic of cartilage cells in knee osteoarthritis
摘要
Abstract
Objective:To investigate the expression and relationship between HSP70 and caspase-3 in knee osteoarthritis. Methods: Forty adult SD rats were divided into experimental group and control group. Thirty rats in experimental group, anterior cruciate ligament transaction (ACLT) was cut off and partial meniscectomy of 1/3 inside incision were performed to reproduce knee osteoarthritis (KOA) model according to Hulth methos,and the other 10 rats was treated with nothing as control group. The rats were sent to the cage and free to move. At 1,2 and 4 weeks later, the arthritis cartilage of femoral and tibial end were observed through immunohistochemistry staining and light microscope. Meanwhile, Mankin scale system was adopted for histomorphology evaluation. Results: Changes of KOA such as hyperplastic synovium, erosion on the surface of cartilage and so on were found in experiment group, the expression of HSP70 was augmentation all the time, but the expression of cas-pase-3 was reduction 1 week later; no similar changes were found in control group. Mankin scale system showed that there were significant differences in the first week as compared with the second week and 4th week (both P<0.01). Conclusion: Heat shock protein inhibit the apoptosis of cartilage cells and protect the cartilage cells in knee osteoarthritis, the conservative treatment for clinical provide objective scientific basis.关键词
骨关节炎,膝/热休克蛋白70/半胱氨酸天冬氨酸蛋白酶-3/软骨细胞/细胞凋亡Key words
Osteoarthritis, knee/ Heat-shock proteins 70/ Caspase-3/ Chrondrocytes/ Apoptosis引用本文复制引用
徐守宇,姚新苗,翟云,潘文胜,方针,何帮剑,许晶虹..膝关节骨关节炎发病过程中HSP70对软骨细胞凋亡的影响[J].中国骨伤,2012,25(10):846-851,6.基金项目
浙江省教育厅高校科研计划基金项目(编号:Y200907379) （编号:Y200907379）
浙江省"重中之重"学科建设经费资助课题(浙教高科[2008]255号) （浙教高科[2008]255号）
