中国骨伤2012，Vol.25Issue(11)：931-936,6.DOI:10.3969/j.issn.1003-0034.2012.11.013
静磁场不同处理时间对体外培养成骨细胞增殖与分化的影响
Effects of static magnetic field at different times on the proliferation and differentiation of osteoblasts in vitro
摘要
Abstract
Objective: To investigate the effect of exposure to static magnetic fields (SMFs) of 3.9 mT on proliferation and differentiation of osteoblasts in vitro. Methods: The newborn rat calvarial osteoblasts were isolated by enzyme digestion and randomly divided into 9 groups after one passage. The intensity of the SMFs was 3.9 mT. The cells were exposed in the SMFs for 0 (control group) ,0.5 ,1.0,1.5 ,2.0,2.5 ,3 ,3.5 and 4.0 h groups respectively. They were observed under the contrast phase microscope each day. After 48 h,cell proliferation was assayed by MTT method. The alkaline phosphatase (Alkaline Phos-phatase.ALP) activities and calcium content were measured after 3,6,9,and 12 days exposed with SMFs. The ALP positive colonies were histochemically stained after 8 days and the calcified nodules were stained by Alizarin Bordeaux after 10 days; BMP-2,Runx-2 and Opg mRNA expression were measured after SMFs treatment in 0,24,48 and 72 h. Results:Contrast with control group,all SMFs groups enhanced cell proliferation (P<0.01 or P<0.05),and they promoted maturation and mineralization of the osteoblasts. The results showed that SMFs improved the ALP activity, promoted calcium content, boost BMP-2,Runx -2 and Opg mRNA expression. Conclusion:The cells exposed to the SMFs of 3.9 mT at 2.5 h apparently promote proliferation and differentiation of osteoblasts in vitro.关键词
成骨细胞/电磁场/细胞增殖/细胞分化Key words
Osteoblasts/ Electromagnetic fields/ Cell proliferation/ Cell differentiation引用本文复制引用
王嘉琪,葛宝丰,马晓妮,周建,郭晓宇,陈克明..静磁场不同处理时间对体外培养成骨细胞增殖与分化的影响[J].中国骨伤,2012,25(11):931-936,6.基金项目
甘肃省科技重大专项资助项目(编号:09ZNKDA025) （编号:09ZNKDA025）
