局解手术学杂志2012，Vol.21Issue(5)：465-469,5.
黄芪和山药配伍治疗MLD-STZ诱导糖尿病小鼠的实验研究
Research of combination therapy with Huangqi and Shanyao on MLP-STZ induced diabetic mice
摘要
Abstract
Objective To study the effects of combination therapy with Huangqi and Shanyao on MLP-STZ(multiple low dose streptozo-tocin MLD-STZ) induced diabetic mice. Methods Mouse model was established by intraperitoneal injection of multiple low dose streptozoto-cin( MLD-STZ). The diabetic mice were divided into 4 groups, and each group was intragastric administered of Shanyao( 1. 4 g ·kg-1 ·d-1) , Huangqi (2.8 g · kg -1 · d -1) , compatible Huangqi with Shanyao (4.2 g · kg -1 · d -1 ) and distilled water (4. 2 g · kg-1 · d -1 ) every day respectively. Detected glucose level and body weight every week,then the diabetic mice were killed after 4 weeks of treatment. Pancreatic histological analysis and islet cell apoptosis were measured by HE and TUNEL labeling. Activities of superoxide dismutase ( SOD) and nitric oxide( NO) in serum of the mice were detected. Proliferation ability of splenocytes to concanavalin A was tested by using MTT assay. The levels of cytokine interferon-γ( IFN-γ) and interleukin- 4( IL- 4)secreted by splenocytes were measured by enzyme linked immunosorbent assay (ELISA). Results Compared with the model group, down-regulation of glucose level and body weight occurred in all medicine treatment groups. The islet cells were protected by Huangqi and Shanyao combination treatment and the apoptosis ratio of this group were significantly decreased. All medicine treatment groups could inhibit NO production. The Shanyao group and combination group up-regulatd serum SOD significantly. The Huangqi group and combination group decreased the proliferation ability of splenocytes to concanavalin A,and down-regulated Thl/Th2 cytokine ratio. Conclusion The results showed that Huangqi and Shanyao combination could effectively prevent diabetic mouse via immunotherapeutic effects and antioxidative effect.关键词
糖尿病/黄芪/山药/配伍/小鼠Key words
diabetic/ Huangqi/ Shanyao/ combination/ mice分类
医药卫生引用本文复制引用
耿淼,陈红艳,仝战旗,王建华,胡亚卓,郝云飞..黄芪和山药配伍治疗MLD-STZ诱导糖尿病小鼠的实验研究[J].局解手术学杂志,2012,21(5):465-469,5.基金项目
“十一五”专项课题(08ZL078) （08ZL078）
