中国临床药理学与治疗学2012，Vol.17Issue(9)：988-995,8.
UPLC-MS/MS法测定Beagle犬血浆中S-奥拉西坦浓度
A sensitive and specific UPLC-MS/MS analysis and preliminary pharmacokinetic characterization of S-oxiracetam in Beagle dogs
汪五三 1季晖 1谢海棠 2戴敏 2贾元威 2梁大虎 2叶雷 3荣祖元3
作者信息
- 1. 中国药科大学药学院,南京,210009,江苏
- 2. 皖南医学院弋矶山医院临床药学部,芜湖241001,安徽
- 3. 重庆东泽医药科技发展有限公司,重庆400030
- 折叠
摘要
Abstract
AIM:To develop a sensitive and specific ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) method for the identification and quantification of S-oxiracetamin in Beagle dog plasma.METHODS:The method involved the addition of piracetam as internal standards,protein-precipitation,UPLC separation,and quantification by MS/MS system using positive electrospray ionization in the multiple reaction monitoring mode (MRM).An HP Amide C18 column (100 mm×3.00 mm,5 μm) was used as the analytical column,while a mixture of methanol-water was used as the mobile phase.The precursor/product ion transitions selected were m/z 159.0/114.1 for S-oxiracetam and m/z 143.0/126.1 for I.S.RESULTS:The lower limit of quantification of S-oxiracetam was 0.05μg/mL.The method was linear in the concentration range of 0.05 - 50 μg/mL.The intra-day and inter-day precisions (RSD) were within 15.0％ for the analytes. S-oxiracetam was proved to be stable during all sample storage,preparation and analytical periods.The method was successfully applied to a pharmacokinetic study in dogs after intragastric administration of S-oxiracetam with a dose of 50 mg/kg.CONCLUSION:The proposed method enables unam biguous identification and quantification for the preliminary pharmacokinetic studies of S-oxirac etam.关键词
S-奥拉西坦/UPLC-MS/MS/药动学/Beagle犬/血浆Key words
S-oxiracetam/ UPLC-MS/MS/Pharmacokinetics/ Beagle dog/ Plasma分类
医药卫生引用本文复制引用
汪五三,季晖,谢海棠,戴敏,贾元威,梁大虎,叶雷,荣祖元..UPLC-MS/MS法测定Beagle犬血浆中S-奥拉西坦浓度[J].中国临床药理学与治疗学,2012,17(9):988-995,8.
