中国临床药理学与治疗学2012，Vol.17Issue(9)：995-1000,6.
槐米中槲皮素提纯、鉴定以及诱导人乳腺癌MCF-7细胞凋亡作用
Effects of quercetins extracted and isolated from flos sophorae immaturus and apoptosis in MCF-7 human breast cancer cell in vitro
摘要
Abstract
AIM: To investigate the effects of quercetins extracted and isolated from the flos sophorae immaturus and apoptosis in MCF-7 human breast cancer cell. METHODS: With the alkali-soluble acid precipitation methods, the quercetins were extracted from flos sophorae immaturus and were identificated by Infrared spec-trophotometry. Colorimetric MTT assay was used to measure the prolification inhibitions of the quercetin in MCF-7 cells. The apoptosis of treated cells were examined by DNA agarose gel electrophoresis analysis of DNA fragmentation and flow cytometry using fluorescein labeling FITC- Annexin V/PI, respectively. RESULTS: MTT results showed that the different doses of quercetin can inhibit the growth of MCF-7 cells in dose- and time-dependent significantly ( P <0. 05). The characteristic bands of apoptosis by DNA electrophoresis were found in MCF-7 cells treated by quercetin at 10. 0 mmol/L for 2 d. The rates of apoptosis of MCF-7 cells which were treated with quercetin at 10. 0 mmol/L for 1 d, 2 d and 3 d were (9.2 ± 1.5)%, (30.0 ± 11.8)% and (60.8±10.6)%, respectively. CONCLUSION; The quercetins can be purified from the flos sophorae immaturus with the alkali-soluble acid precipitation methods. The quercetin can inhibit the growth and apoptosis in MCF-7 cells.关键词
槲皮素/槐米/人乳腺癌MCF-7细胞/诱导凋亡Key words
Quercetin/ Flos sophorae immaturus/ MCF-7 cell/ Apoptosis分类
医药卫生引用本文复制引用
张海英,徐水凌,张力衡..槐米中槲皮素提纯、鉴定以及诱导人乳腺癌MCF-7细胞凋亡作用[J].中国临床药理学与治疗学,2012,17(9):995-1000,6.基金项目
浙江省中医药科技计划项目(2008CA108) （2008CA108）
