中国临床药理学与治疗学2012，Vol.17Issue(9)：1007-1009,3.
非免疫性过敏反应细胞模型的建立
Anaphylactoid reaction based on RBL -2H3 cell model in vitro
摘要
Abstract
AIM: To observe the effects of C48/80 on RBL-2H3 cell degranulation orthogonal test, optimize the experimental conditions and discuss the possible detection methods of anaphylactoid reaction . METHODS: The β-hex-osaminidase assay of RBL-2H3 cells incubated with C48/80 were tested by orthogonal test considering two laboratorial factors time and cell concentration. According the results the optimized cell model in vitro were set up and disscus the superior and the inferior aspects between different detection means. RESULTS: Different concentrations and time could make significance difference on RBL-2H3 cell model with degranulation. CONCLUSION: Anaphylactoid reaction based on RBL -2H3 cell model in vitro could be buill up on the cell concentration of 2 × 105 under 60 minutes incubation time , which may be appropriate conditions in order to get stable cell model because the degranualtion was significantly increased and dose-dependent under this condition.关键词
类过敏反应/RBL-2H3细胞/脱颗粒/正交试验Key words
Anaphylactoid reaction/ RBL-2H3 cell/ Degranulation/ Orthogonal test分类
医药卫生引用本文复制引用
赵吟,李钦,郑晓亮,张信岳..非免疫性过敏反应细胞模型的建立[J].中国临床药理学与治疗学,2012,17(9):1007-1009,3.基金项目
浙江省科技厅面上项目资助(2009C33033) （2009C33033）
浙江省自然科学基金(C11007D) （C11007D）
