中国临床药理学杂志2012，Vol.28Issue(9)：662-664,3.
脂联素的球状结构域对3T3-L1脂肪细胞胰岛素抵抗模型AMPK信号通路的影响
Effect of globular domain of adiponectin on glucose metabolism and its mechanism in 3T3-L1 adipocytes model which have generated IR
摘要
Abstract
Objective To observe the effect of globular domain of adi-ponectin on glucose metabolism and its mechanism in 3T3 - LI adipo-cytes model which have generated insuline resistance ( IR). Methods Culturing and differentiating 3T3 - LI adipocyte. IR model of 3T3 - LI adipocytes were prepared with palmitic acid. Intervention the adipocytes which have been generated IR with different concentrations of gAd. The cell culture medium glucose content was detected with the glucose oxidize method. The mRNA expression of AMPK, CPT - I were detected with real - time quantitative PCR method. The phosphorylation of AMPK Thr -172 was detected by Western blot. Results Gad can promote glucose uptake of 3T3 - LI adipocytes model which have generated IR. Different concentrations of gAd gradually increased the mRNA expression levels of AMPK, CPT - I and in a dose - dependent manner. The AMPK Thr -172 phosphorylation levels increased gradually followed by the concentration of gAd ( P = 0. 000 ). Conclusion gAd can improve insulin resistance by AMPK signal transduction in 3T3 - LI adipocytes model which have generated IR.关键词
胰岛素抵抗/脂联素球状结构域/3T3-L1脂肪细胞Key words
insuline resistance/ globular domain of adiponectin/ 3T3 -LI adipocytes分类
医药卫生引用本文复制引用
王彦,陈思思,廉如,高婷婷,陈显久,杨静..脂联素的球状结构域对3T3-L1脂肪细胞胰岛素抵抗模型AMPK信号通路的影响[J].中国临床药理学杂志,2012,28(9):662-664,3.基金项目
山西省科技攻关基金资助项目(20090321104) （20090321104）
