中国临床药理学杂志2012，Vol.28Issue(10)：773-775,3.
LC-MS/MS法测定人粪便中匹诺塞林的浓度及在粪便排泄中的应用
Determination of pinocembrin in human feces by LC-MS/MS and and its application to feces excretion
摘要
Abstract
Objective To develop an LC - MS/MS method for the determination of pinocembrin in human feces and apply the method to the study of feces excretion. Methods The column was a Phenomenex Luna? C8 column and the mobile phase consisted of acetonitrile - ammonium acetate aqueous solution (65: 35) at a flow rate of 0. 25 mL ? Min-1. Acetonitrile was used in sample preparation. The detection was performed by multiple reaction monitoring mode via a negative eletrospray ionization source. Results The assay was linear over the range of 30 - 4000 ng ? mL -1 with a limit of quantitation of 30 ng ? mL-1, the RSD of intra -and inter-day validation were both less than 7% , the recovery was all greater than 90% , and there was no matrix effects. Feces excretory rate in 48 h was (1.9 ±1.4)%. Conclusion This validated assay is a rapid , sensitive, selective and reliable method for the determination of pinocembrin in human feces, which provided a base for the study on feces excretion of pinocembrin.关键词
匹诺塞林/LC-MS/MS/粪便Key words
pinocembrin/ LC - MS/MS/ feces分类
医药卫生引用本文复制引用
孙韬华,严蓓,曹国颖,李可欣,闫记灵,宋艳芳,王唯红,胡欣..LC-MS/MS法测定人粪便中匹诺塞林的浓度及在粪便排泄中的应用[J].中国临床药理学杂志,2012,28(10):773-775,3.基金项目
国家科技部"重大新药创制"科技重大专项心脑血管疾病新药临床评价技术平台研究基金资助项目(2012ZX09303008-002) （2012ZX09303008-002）
