中国临床药学杂志2012，Vol.21Issue(5)：276-280,5.
1,25-二羟维生素D3对其诱导的树突状细胞上Toll样受体7表达的抑制作用
Effect of 1, 25-dihydroxyvitamin D3 to inhibit dendritic cells maturation through down-regulation of Toll-like receptor 7
摘要
Abstract
AIM To explore the possible role of Toll-like receptor 7(TLR7) in the procedure of the inducement of immature dendritic cells( DCs) by 1,25-dihydroxyvitamin D3. METHODS DCs were harvested by differentiating mouse borrow cells in. vitro. The influence of 1, 25-dihydroxyvitamin D3 on phenotype and function of DCs was determined by flow cytometry and mixed lymphocyte reactions. The expression of TLR7 on 1,25-dihydroxyvitamin ^-induced DCs was determined by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR.). RESULTS Treated by 1, 25-dihydroxyvitamin D3, DCs had significantly lower fluorescence intensity of CD80 and MHCII ( P ＜ 0.05), and DCs' ability to stimulate T cells was significantly lower than the control( P ＜ 0.05) . Compared to the control, 1,25-dihydroxyvitamin D3 could obviously down-regulate the expression of TLR7(P ＜ 0.05) . CONCLUSION 1,25-Dihydroxyvitamin D3 can inhibit the maturation of DCs, and might make DCs tolerant by influencing the expression of TLR7.关键词
树突状细胞/1,25-二羟维生素D3/Toll样受体7Key words
dendritic cell/ 1,25-dihydroxyvitamin D3/ Toll-like receptor 7引用本文复制引用
刘飞,杨明珍,李庆生,宋玲,夏雷鸣,李嘉嘉..1,25-二羟维生素D3对其诱导的树突状细胞上Toll样受体7表达的抑制作用[J].中国临床药学杂志,2012,21(5):276-280,5.基金项目
安徽省自然科学基金(编号1208085MH154) （编号1208085MH154）
