中国美容医学2012，Vol.21Issue(11)：1525-1527,3.
骨髓来源血管内皮祖细胞的体外分离培养和鉴定
Bone marrow-derived endothelial progenitor cells isolation and culture
摘要
Abstract
Objective To isolate and culture endothelial progenitor cells (EPCs). Methods Bone marrow cell were cultured in dishes coated with 1% gelatin with M199 medium supplemented with 10% fetai bovine serum and 50μg/mL endothelial cell growth supplements,at 37°C in a humidified atmosphere of 5% CO2. Subcultured using trypsin-EDTA. EPCs were identified by immunofluorescence staining for CD31 expression, ability of lectin binding. Further characterisation of EPCs was performed by analysing capillary tube formation. Results EPCs colonies appeared between 5 and 7 days of culture and defined as a central core of rounded cells. Two weeks later, cells presented the characteristic cobblestone morphology. The differentiation status of EPCs was confirmed by binding UEA-1 plant lectin and expressing CD31. Further, tubular network was formed. Conclusions When induced in culture, EPCs can be obtained, exhibit long term proliferative potential.关键词
血管内皮祖细胞/骨髓/分化Key words
endothelial progenitor cells/ bone marrow/ differentiation分类
生物科学引用本文复制引用
张蓉,高瞻,郭增良,王荣耀,邓炜焯,刘彦普..骨髓来源血管内皮祖细胞的体外分离培养和鉴定[J].中国美容医学,2012,21(11):1525-1527,3.基金项目
新疆维吾尔自治区自然科学基金项目(2012211A040) （2012211A040）
