中国美容医学2012，Vol.21Issue(11)：1536-1538,3.
当归挥发油对增生性瘢痕成纤维细胞增殖、凋亡及胶原合成的影响
Effect of angelicanaphtha on proliferation, apoptosis, collagen synthesis of human hypertrophic scar fibroblast
刘凯 1张选奋 1张瑾 1秦永红 1钟琳1
作者信息
- 1. 兰州大学第二医院整形美容科 甘肃兰州730030
- 折叠
摘要
Abstract
Objective To investigate the effects of Angelicanaphtha on proliferation cell cycle, apoptosis, and collagen synthesis of human hypertraphic scar fibroblast. Methods Human hypertrophic scar fibroblast was cultured and passaged in Dulbecco's modified Eagle's medium (DMEM) and treated with Angelicanaphtha 1mg/L, 4mg/L, and 16mg/L at 1, 3, 5, and 7day respectively. The proliferation was measured with MTT method. The cell cycle and apoptosis were analyzed with Flow cytometry and collagen synthesis was determined with radioimmunoassay. Results Angelicanaphtha of 1mg/L, 4mg/L, and 16mg/L made G0/G1 phase cells, G2/ M phase cells decrease and S phase cells remarkable increase (P<0.05). Furthermore, apoptosis of the fibroblasts was increased with increase of Angelicanaphtha concentration (P<0.05). The collagen synthesis of fibroblast was inhibited by Angelicanaphtha in concentration-dependent manner (P<0.05). Conclusion The proliferation and collagen synthesis of fibroblast was inhibited by Angelicanaphtha.关键词
当归挥发油/成纤维细胞/增殖/凋亡/胶原合成Key words
angelicanaphtha/ fibroblasts cells/ proliferation/ apoptosis/ collagen synthesis分类
生物科学引用本文复制引用
刘凯,张选奋,张瑾,秦永红,钟琳..当归挥发油对增生性瘢痕成纤维细胞增殖、凋亡及胶原合成的影响[J].中国美容医学,2012,21(11):1536-1538,3.
