中国美容医学2012，Vol.21Issue(15)：1961-1964,4.
氨甲喋呤对人增生性瘢痕成纤维细胞增殖和胶原合成的影响
Effects of methotrexate on proliferation and collagen synthesis of hypertrophic scar fibroblast
张建军 1陈发国 2吴毅平3
作者信息
- 1. 华中科技大学同济医学院附属同济医院整形外科 湖北 武汉 430030
- 2. 石河子大学医学院第一附属医院烧伤整形科 新疆 石河子 832008
- 3. 乌鲁木齐职业病防治医院整形外科 新疆 乌鲁木齐 832000
- 折叠
摘要
Abstract
Objective The paper explore the effects of Methotrexate on proliferation and collagen synthesis of fibroblast derived from human hypertrophic scar. Methods Scar -derived human fibroblasts were cultured in vitro, the hypertrophic scar(HS) fibroblast (from the 4th to 6th passages were treated with various concentrations of Methotrexate, cell culture solution as negative control, toxic reaction of Methotrexate on HS fibroblast was examined by lactate dehydrogenase (LDH) activity. The effects of Methotrexate on HS fibroblast proliferation and collagen synthesis were examined by MTT colorimetric cell proliferation assay and Hydroxyproline (Hrp) determination. Results Firstly, Methotrexate with 10-6mol/L and 10-5mol/L doses significantly inhibited proliferation hypertrophic scar fibroblasts, compared with control group (P<0.01). Secondly, Methotrexate with 10-7mol/L～10-5mol/L doses had cytotoxicity on hypertrophic scar fibroblasts (P<0.05).Thirdly, Methotrexate with 10-9mol/L～10-5mol/L doses inhibited collagen synthesis of hypertrophic scar fibroblasts, compared with control group (P<0.01). Conclusion Methotrexate can significantly inhibits the proliferation and collagen synthesis of HS fibroblast in vitro, which may have some effect on prevention and treatment of HS.关键词
氨甲喋呤/增生性瘢痕/成纤维细胞/细胞增殖/胶原合成Key words
methotrexate/ hypertrophic scar /fibroblast/ proliferation/ collagen synthesis分类
医药卫生引用本文复制引用
张建军,陈发国,吴毅平..氨甲喋呤对人增生性瘢痕成纤维细胞增殖和胶原合成的影响[J].中国美容医学,2012,21(15):1961-1964,4.
