中国美容医学2012，Vol.21Issue(15)：1967-1971,5.
EGCG对中波紫外线辐射后原代人角质形成细胞中光产物产生和清除的影响
Effections of epigallocatechingallate on production and removal of photoproducts induced by UVB irradiation in human primary keratinocyte cells
林向飞 1闵玮 2朱晓芳 1骆丹3
作者信息
- 1. 扬州大学临床医学院江苏省苏北人民医院皮肤科 江苏 扬州 225001
- 2. 苏州大学附属第一医院皮肤科 江苏 苏州215006
- 3. 南京医科大学附属第一医院皮肤科
- 折叠
摘要
Abstract
Objective We investigated the production and removal process of photoproducts (CPDs) induced by UVB irradiation and the intervention effect of epigallocatechingallate (EGCG) in human primary keratinocyte (KC). Methods Human primary KC were cultured with or without 200|jg/ml EGCG and were irradiated by different dosages of UVB. The production and removal of CPDs at different time intervals and different dosages were examined by immunohistochemical method. Results CPDs appeared in human primary KC after 30mJ/cm2 UVB irradiation and reached the peak at 1h and removed rapidly during the first 4h and then removed slowly or almost cleared out up to 24h after UVB irradiation. The quantity of CPDs was decreased when irradiated human primary KC were incubated with EGCG (P<0.05). Conclusions The photodamage of human primary KC was UVB dose-dependent. EGCG can decrease the level of CPDs and rapid the removal of CPDs.关键词
UVB辐射/人原代角质形成细胞/环丁烷嘧啶二聚体/EGCGKey words
UVB irradiation/ human primary keratinocyte/ CPDs/epigallocatechingallate(EGCG)分类
生物科学引用本文复制引用
林向飞,闵玮,朱晓芳,骆丹..EGCG对中波紫外线辐射后原代人角质形成细胞中光产物产生和清除的影响[J].中国美容医学,2012,21(15):1967-1971,5.
