中国免疫学杂志2012，Vol.28Issue(10)：918-921,4.DOI:10.3969/j.issn.1000-484X.2012.10.012
宫颈癌细胞通过分泌TSLP促进自身细胞活力
Human thymic stromal lymphopoietin promotes the viability of cervical cancer cells
摘要
Abstract
Objective:To explore whether thymic slromal lymphopoietin (TSLP) regulates the viability of cervical cancer cells. Methods: We cultured HeLa and CasKi cells for 24, 48 and 72 h in vitro, and collected the supernatants for analyzing the secretion of TSLP by ELISA, respectively. Flow cytomelry was performed to delect the expression of TSLPR on HeLa and CasKi cells. Moreover, we used MTT assay to analyze the viability of HeLa and CasKi cells after trealment with recombinant human TSLP at differenl concentration (1,10 and 100 ng/ml). Results;Both HeLa and CasKi cells secreled TSLP in a time-dependent manner (P <0. 01) , and the TSLP secretion level of HeLa cells was higher than that of CasKi cells at 24 and 48 h (P <0. 01 or P <0. 05). The ratio of TSLPR positive HeLa and CasKi cells was (23.61 ±1.30) and (27.07 ±2. 13) , respectively (P<0.05). Furthermore, TSLP at the concentration with 10 or 100 ng/ml can promole the viability of HeLa and CasKi cells after 48 and 72 h. Conclusion: TSLP could up-regu-lale the viability of cervical cancer cells, and thus contributes lo the growth of cervical cancer calls, which may be involved in the progression of cervical cancer.关键词
胸腺基质淋巴细胞生成素(TSLP)/宫颈癌/HeLa/CasKi/细胞活力Key words
Thymic slromal lymphopoielin/ Cervical cancer/ HeLa/ CasKi/ Cell viability分类
医药卫生引用本文复制引用
谢锋,孟玉菡,李明清,李大金..宫颈癌细胞通过分泌TSLP促进自身细胞活力[J].中国免疫学杂志,2012,28(10):918-921,4.基金项目
本文受国家自然科学基金重点项目(No.30730087)及上海市医学领军人才基金项目资助 （No.30730087）
