农学学报2012，Vol.2Issue(10)：9-13,5.
花生体胚诱导及植株再生
Somatic Embryogenesis and Plant Regeneration in Peanut
摘要
Abstract
Efficient somatic embryogenesis and plant regeneration in embryonic leaflet culture of peanut (Arachis hypogaea L.) were studied, for providing the methods of somatic hybridization, In vitro mutagenesis and genetic transformation. The embryonic leaflets of 17 peanut cultivars in 5 types were cultured on MSB5 medium (Murashige and Skoog salts plus B5 vitamins) supplemented with 10.0 mg/L 2,4-D to induce somatic embryos. After 4 weeks culture, the explants with somatic embryos were transferred to the medium supplemented with 4 mg/L BAP for somatic embryo germination and plant regeneration. The result showed that the frequencies of somatic embryo induction and plant regeneration were different significantly in 5 peanut types. Intermediate type gave a higher frequency (84.1%-91.2%) of somatic embryo induction and a higher frequency (81.1%-97.0%) of plant regeneration. The frequencies of somatic embryo induction were significantly different among the cuhivars in Arachis hypogaea var. vulgaris with a range from 43.9% to 85.0% and Arachis hypogaea var. hypogaea with a range from 42.2%-82.2%, respectively, but the higher frequencies of plant regeneration were obtained in them, sharing a range from 94.3%-96.5% and 88.1%-98.7%,respectively. Araehis hypogaea var. fastigiata showed the lowest frequencies of somatic embryo induction (32.2%-50.0%) and plant regeneration (41.2%-55.1%). The frequencies of somatic embryo induction (61.1%-87.8%) and plant regeneration (63.7%-87.6%) were obtained in Araehis hypogaea vat. hirsute. When the plantlets were grafted onto germinated seedlings and transplanted to the field, they grew to maturity and produced viable seeds. The result showed that the regeneration capacity was different among various types of peanut, and the regeneration capacity of Araehis hypogaea var. fastigiata was the lowest.关键词
花生/胚小叶/胚胎发生/植株再生Key words
Peanut (Aracbis hypogaea L.)/Embryonic Leaflet/Somatic Embryogenesis/Plant Regeneration分类
农业科技引用本文复制引用
乔利仙,隋炯明,潭玲玲,郭宝太,孙世孟,王晶珊..花生体胚诱导及植株再生[J].农学学报,2012,2(10):9-13,5.基金项目
山东省自然基金“花生离体诱变及创造抗旱耐盐种质资源研究” （）
国家自然基金青年基金“花生几丁质酶基因、β-1,3-葡聚糖酶基因启动子的克隆与功能分析”（31101178）：山东省自然基金青年基金“花生维生素E合成关键基因的克隆及其抗氧化机制研究” （）
山东省中青年科学家奖励基金“花生Chi基因及Glu基因的克隆、双价表达载体的构建及遗传转化研究”（Bs2009NY028）. （）
