中国农业科学2012，Vol.45Issue(18)：3677-3687,11.DOI:10.3864/j.issn.0578-1752.2012.18.001
优质面条商品小麦澳白麦相关品质基因的分子标记鉴定
Molecular Marker Identification of Noodle Quality Related Genes in a Commercial Noodle Wheat from Australia
摘要
Abstract
[Objective] A commercial noodle wheat from Australia, known as Australian Standard White in China due to its top noodle quality, was a mixture of several genotypes. Noodle quality related genes were identified for providing information of noodle quality improvement to wheat breeding in China. [Method] A total of 36 selected lines from Australian Standard White population were analyzed with 38 functional markers including high molecular weight glutemn subunits (HMW-GS), low molecular weight glutenin subunits (LMW-GS), 1B/1R translocation, waxy protein subunits, polyphenol oxidase (PPO) activity, yellow pigment content, grain hardness and resistance to pre-harvest sprouting (PHS). [Results] In the 36 lines, there were 13 genes with positive effects on noodle quality, among which non-]B/lR translocation type and HMW-GS gene Bx7 covered 100%, whereas HMW-GS gene By8, LMW-GS genes Glu-A3b and Glu-Bib, Wx-B1 null type, low PPO activity allele Ppo-D1a, low yellow pigment allele Psy-B1b, soft to medium grain hardness allele Pinb-D1a and PHS resistant gene Vp-1Bc had frequencies over 80%. Out of the 36 lines, 12 genotypes were found, among which genotype 2 predominated in the highest frequency up to 63.9%, which covered the most of refered positive genes such as HMW-GS genes Bx7 and By8, LMW-GS genes Glu-A3b and Glu-B3b, low PPO activity gene Ppo-D1a, low yellow pigment content gene Psy-B1b, soft to medium hardness gene Pinb-D1a, PHS resistant gene Vp-1Bc, non-1B/1R translocation and Wx-B1 null type, and had the least of refered negative genes such as high PPO activity gene Ppo-A1a and high yellow pigment content gene Psy-A1a. [Conclusion] Australian Standard White was a commercial wheat composed of several cultivars. Pyramiding most of positive noodle quality genes in cultivars and mixing the cultivars in the end-use quality needed proportion resulted in its high noodle quality. For a noodle wheat breeding plan, genotype 2 might be the most promising candidate of noodle quality donors in Australian Standard White.关键词
澳白麦/面条品质/品质基因/分子标记Key words
Australian Standard White/ noodle quality/ quality gene/ molecular marker引用本文复制引用
李式昭,伍玲,郑建敏,朱华忠..优质面条商品小麦澳白麦相关品质基因的分子标记鉴定[J].中国农业科学,2012,45(18):3677-3687,11.基金项目
四川省财政基因工程青年基金项目、四川省农作物育种攻关项目、科技部国合项目(2011DFG33350)、国家"十二五"科技支撑计划小麦新品种培育与扩繁(2011BAD35B03)、国家"863"计划项目(2012AA101105) （2011DFG33350）
