中国农业科学2012，Vol.45Issue(18)：3744-3754,11.DOI:10.3864/j.issn.0578-1752.2012.18.007
马铃薯甲虫病原细菌分离及生防菌的筛选与鉴定
Isolation, Screening and Identification of Bacterial Agents for Biological Control of Colorado Potato Beetle (Leptinotarsa decemlineata)
摘要
Abstract
[Objective] The objective of this study is to isolate and screen bacterial biocontrol agents against Colorado potato beetle (CPB) (Leptinotarsa decemlineata). [Method] Diseased CPB adult samples were collected from Xinjiang and bacterial isolates were obtained from the samples. These isolates were tested for CPB pathogenicity. Highly insecticidal isolates were screened through laboratory and field assays and identified morphologically and molecularly. [ Result ] A total of 126 bacterial isolates were obtained from diseased adults of CPBs collected from Xinjiang Autonomous Region of China and 36 isolates showed protein- and chitin-degrading activity. Among these isolates, 11 infected 1st instar and 2nd instar CPB larvae and resulted in lethal rates of 21.0%-77.9% within 7 days after inoculation when tested on detached potato leaves in the laboratory. The 11 infectious isolates were selected for field tests and 6 of them induced high CPB mortality rates. The CPB-lethal rates by the 3 isolates (CPB008, CPB012 and CPB016) reached 41.0%-49.9%. The other 3 isolates (CPB072, CPB108 and CPB111) also had significantly high CPB lethal rates (21.1%-37.8%). CPB008, CPB012, CPB016 and CPB111 were identified as Bacillus thuringiensis and CPB072, CPB108 were B. atrophaeus, respectively. [Conclusion] The screened 3 isolates (CPB008, CPB012 and CPB016) have strong CPB pathogenicity,and could be potential applications in biological control of this destructive pest in Xinjiang of China.关键词
马铃薯甲虫/苏云金芽孢杆菌/萎缩芽孢杆菌/生物防治/杀虫效率Key words
Colorado potato beetle/ Bacillus thuringiensis/ Bacillus atrophaeus/ biological control/ insecticidal efficiency引用本文复制引用
罗华东,严加林,余洋,谭万忠..马铃薯甲虫病原细菌分离及生防菌的筛选与鉴定[J].中国农业科学,2012,45(18):3744-3754,11.基金项目
国家公益性行业(农业)专项(201103027-3) （农业）
