中国农业科学2012，Vol.45Issue(18)：3841-3848,8.DOI:10.3864/j.issn.0578-1752.2012.18.017
腺苷甲硫氨酸对成肌细胞成脂分化及脂肪沉积的影响
Effects of S-Adenosylmethionine (SAM) on Adipogenic Differentiation and Lipid Accumulation of C2C12 Cells
摘要
Abstract
[Objective] This experiment was conducted to investigate the effects of S-adenosylmethionine (SAM) on adipogenesis and lipogenesis of C2C12 mouse myoblast. [Method] Cells were cultured in high glucose DMEM medium containing 0 mmol·L-1, 0.25 mmol·L-1, 1.0 mmol·L-1, and 2.0 mmol·L-1 SAM. After treated for 0 h, 24 h, 36 h, and 48 h, cells were stained with Red Oil O and observed under microscope. The lipid accumulation in cytosol was determined by optical density (OD) measurement. After being treated for 2 d, cells were harvested for collection of total RNA and protein. Then RT-PCR and western blotting approaches were used to determine mRNA and protein expression levels of several genes related with adipogenesis and lipogenesis. [Result! The results showed that, compared with the control group, C2C12 cells presented typical features of adipocytes when observed under microscope after treated with SAM. SAM also increased the number of lipid droplets in the cells. In addition, the increasing trend became more significant when cells were treated with higher concentration of SAM. At the same time, mRNA and protein expression levels of key regulator of differentiation of adipocytes, PPARy and C/EBPa, were elevated (P<0.05) after treatment. mRNA expression levels of other lipogenesis related genes, such as aP2, FAS and SREBP-1, were also elevated markedly. Compared with the control group, the relative mRNA levels of these three genes were increased by 6.86 (P<0.01), 3.45 (P<0.05), and 3.48 (P<0.01) folds, respectively, in 2.0 mmol-L"1 group. [Conclusion] The results indicated that SAM had the ability to promote adipogenic differentiation and lipid accumulation of C2C12 cells.关键词
小鼠/成肌细胞/腺苷甲硫氨酸/成脂分化/脂肪沉积Key words
mouse/ skeletal muscle fibroblast/ SAM/ adipogenic differentiation/ lipid accumulation引用本文复制引用
于渤洋,吕文涛,巨婷婷,刘洋,姜美华,尹靖东..腺苷甲硫氨酸对成肌细胞成脂分化及脂肪沉积的影响[J].中国农业科学,2012,45(18):3841-3848,8.基金项目
国家自然科学基金项目(30972113)、国家"973"计划项目(2012CB124702)、重庆市重点实验室专项经费自主科研课题 （30972113）
