中国农业科学2012，Vol.45Issue(18)：3859-3872,14.DOI:10.3864/j.issn.0578-1752.2012.18.019
高致病性PRRSV与PCV2共感染协同致病性研究
Studies on Synergetic Pathogenicity of Co-infection with Highly Pathogenic PRRSV and PCV2
摘要
Abstract
[Objective] The purpose is to study the synergetic pathogenic effect between highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) and porcine cirovirus type 2 (PCV2), and to clarify the relation of ordinal co-infection and synergetic pathogenicity. [Method] Thirty 35-day-old piglets were randomly selected and separated into 6 groups which designated PCV2/HP-PRRSV ordinal co-infection group, HP-PRRSV/PCV2 ordinal co-infection group, HP-PRRSV+PCV2 simultaneous co-infection group, HP-PRRSV infection group, PCV2 infection group and control group, respectively. Clinical symptom and temperature were recorded everyday post-inoculation, meanwhile, weighing body weight and collecting blood samples. Variations of CD3+CD4+CD8-, CD3+CD4-CD8+, γδT, NK, granulocyte and mononuclear cell were determined by flow cytometry. Two kinds of antibodies against HP-PRRSV and PCV2 were quantified by immunoperoxidase monolayer assay (IPMA). Concentrations of IFN-γ, TNF-α, IL-10, IL-2, GM-CSF in serum were determined by ELISA. Pathological changes were observed through making pathological section for different organs. [ Result ] All the findings suggest that the pathogenicity of HP-PRRSV/PCV2 ordinal co-infection is conspicuously more severe than other testing groups. Mortality of this group is 60% surprisingly. Virus load in body is the highest while antibodies level is the lowest. Variation of immunocyte subgroups and cytokines (especially TNF-α) is the most conspicuous. [Conclusion] There is a synergetic pathogenicity of co-infection of pigs with HP-PRRSV and PCV2. If HP-PRRSV infecting precedes PCV2, the mortality and mortality will be conspicuously enhanced. This study has provided a scientific proof to control the two viruses.关键词
高致病性猪繁殖与呼吸综合征病毒/猪圆环病毒2型/共感染/协同致病性Key words
highly pathogenic porcine reproductive and respiratory syndrome virus/ porcine cirovirus type 2/ co-infection/ synergetic pathogenicity引用本文复制引用
范培虎,危艳武,郭龙军,吴洪丽,黄立平,刘长明..高致病性PRRSV与PCV2共感染协同致病性研究[J].中国农业科学,2012,45(18):3859-3872,14.基金项目
国家自然科学基金项目(30871859)、农业科技成果转化基金项目(2010GB23260563) （30871859）
