中国农业科学2012，Vol.45Issue(18)：3892-3898,7.DOI:10.3864/j.issn.0578-1752.2012.18.022
Q型烟粉虱化学感受蛋白基因BtabCSP1的克隆与分析
Cloning and Characteristics Analysis of Chemosensory Protein Gene BtabCSP1 in Bemisia tabaci Q Biotype
摘要
Abstract
[Objective] The objective of this study is to clone the full length cDNA of the gene encoding chemosensory proteins from Bemisia tabaci Q biotype, and to analyze its expression pattern at different developmental stages. [Method] Based on the amino acid sequence of aphids (Myzus persicae) chemosensory proteins OS-D2a (ABM5558) reported previously, a pair of primers were designed based on simulation of gene splicing and RT-PCR was used to clone the chemosensory protein gene BtabCSPI. [Result] Sequencing and structural analysis showed that BtabCSPI contains a 2 626 bp long complete reading frame (with 2 exons and 1 intron) and encodes 131 amino acid residues with a CX6CX18CX2 domain, the typical characteristics of a chemosensory protein gene. As displayed by semi-quantitative RT-PCR amplification from total RNA of B. tabaci of various stages, BtabCSPI was expressed in all samples and the expressions were more apparent in the 2nd instar, 3rd instar and 4th instar (pupal stage) nymphs. The amino acid (encoded by BtabCSPI) sequence cluster analysis showed that B. tabaci was distantly related to fruit flies and aphids and chemosensory protein gene was active in the process of species evolution. [ Conclusion ] BtabCSPI was isolated from B. tabaci Q biotype, and the expression pattern suggested the close relationship between the expression of this gene and the development of B. tabaci.关键词
Q型烟粉虱/化学感受蛋白/基因克隆/特征分析Key words
Bemisia tabaci Q biotype/ CSP/ gene cloning/ characteristics analysis引用本文复制引用
白润娥,李静静,唐雅菲,熊大斌,李帅良,闫凤鸣..Q型烟粉虱化学感受蛋白基因BtabCSP1的克隆与分析[J].中国农业科学,2012,45(18):3892-3898,7.基金项目
国家转基因新品种培育重大专项项目(2009ZX08012-007B) （2009ZX08012-007B）
