中国普通外科杂志2012，Vol.21Issue(10)：1231-1235,5.
白细胞介素1α及其受体拮抗剂对大肠癌细胞增殖、侵袭的影响
Effect of interleukin 1α and its receptor antagonist on proliferation and invasion of human colon cancer cell lines
史新龙 1马家驰 2杨熊飞 2顾远晖 2李一平2
作者信息
- 1. 宁夏医科大学研究生院,宁夏银川750004
- 2. 甘肃省人民医院普通外科,甘肃兰州730000
- 折叠
摘要
Abstract
Objective: To investigate the effects of interleukin lα (IL-Iα) and its receptor antagonist (IL-Ira) on the proliferation and invasion of human colon cancer cells with different metastatic potentials as well as the role of IL-lα and IL-Ira in metastatic process.Methods: The colonic carcinoma cell lines with high liver metastatic potential (WiDr) and low liver metastatic potential (Caco-2 and Colo-320) were used. The gene and protein expressions of IL-la and its type I receptor (IL-1R I) were determined by RT-PCR and ELSIA assay, respectively. After exposure to different concentrations of the exogenous IL-la and IL-lra, the cell proliferation of the three cell lines was measured by WSTM assay and the invasive ability of WiDr and Caco-2 cells was assessed by using Transwell chamber. Results: The RT-PCR results showed that the IL-lα mRNA was only expressed in the WiDr cells of high liver metastatic potential but not in Caco-2 and Colo-320 cells of low liver metastatic potential, while the IL-1RI gene was expressed in all the three types of cells. The concentration of IL-lα protein detected in WiDr cells by ELISA assay was (7.85±0.52) pg/mL but was not detected in Caco-2 and Colo-320 cells. The exogenous IL-lα significantly increased the proliferative ability of Caco-2 and Colo-320 cells (all P<0.05), but had no effect on that of WiDr cells (P>0.05)} while the exogenous IL-lra markedly inhibited the proliferation of WiDr cells (P<0.05). The exogenous IL-lα significantly enhanced the invasive ability of Caco-2 cells (P<0.05), but had no influence on that of WiDr cells (P>0.05); the exogenous IL-lra significantly inhibited the invasiveness of WiDr cells (P<0.05), but had no effect on Caco-2 cells (P>0.05). Conclusion: The liver metastatic potential of colon cancer is related to its IL-lα expression; and exogenous IL-lra can inhibit the proliferative and invasive ability of colon cancer cells.关键词
结肠肿瘤/白细胞介素1α/受体,白细胞介素/肿瘤侵润Key words
Colonic Neoplasms/ Interleukin-1a/ Receptors, Interleukin/ Neoplasm Invasiveness分类
医药卫生引用本文复制引用
史新龙,马家驰,杨熊飞,顾远晖,李一平..白细胞介素1α及其受体拮抗剂对大肠癌细胞增殖、侵袭的影响[J].中国普通外科杂志,2012,21(10):1231-1235,5.
