中国普通外科杂志2012，Vol.21Issue(10)：1240-1243,4.
胃转流术对GK大鼠肝脏脂代谢的影响及其意义
Effect of gastric bypass surgery on hepatic lipid metabolism in GK rats and its significance
摘要
Abstract
Objective: To investigate the effect of gastric bypass procedure on hepatic lipid metabolism in spontaneous diabetic Goto-Kakizaki (GK) rats and its significance. Methods: Forty male GK rats were equally randomized into experimental group and control group. Rats in experimental group underwent Roux-en-Y gastric bypass operation while rats in control group underwent antroduodenal transaction and then, reanastomosis in situ. The fasting glucose and 2-h oral glucose tolerance test (2-h OGTT) glucose value of the two groups were measured before and 1 week, 2 and 4 weeks after surgery respectively, and the fasting blood levels of free fatty acids (FFA), total cholesterol (Tch) and triglyceride (TG) of rats before and 4 weeks after surgery were also measured. In addition, liver tissues of rats obtained intraoperatively and 4 weeks after surgery were used for immunohistochemical oil red O staining to observe the TG deposits in the liver. Results: Compared with those before surgery, the values of both fasting glucose and 2-h OGTT glucose showed a gradually declining trend after surgery in experimental group, which decreased significantly at 2 and 4 weeks after surgery (all P<0.05); in experimental group, the blood FFA level significantly decreased (P<0.05), while there were no significant changes in the TG and Tch level (both P>0.05); but the TG accumulation in the liver tissue section was markedly reduced. All of the above parameters and measurements in control group showed no obvious changes after surgery compared with preoperative values (all P>0.05), Conclusion: The blood sugar-lowering effect of gastric bypass procedure may be probably associated with its improving hepatic lipid metabolism and decreasing FFA concentration, thereby ameliorating the insulin resistance.关键词
糖尿病,2型/外科学/胃转流术/脂代谢障碍Key words
Diabetes Mellitus, Type 2/surg/ Gastric Bypass/ Lipid Metabolism Disorders分类
医药卫生引用本文复制引用
邹洪,闫洪涛,伍松,唐均成,郑晓博,崔建峰,石力,陈涛,汤礼军..胃转流术对GK大鼠肝脏脂代谢的影响及其意义[J].中国普通外科杂志,2012,21(10):1240-1243,4.基金项目
全军临床高新技术重大项目(2010gxjs040). （2010gxjs040）
