中国全科医学2012，Vol.15Issue(32)：3734-3736,3.DOI:10.3969/j.issn.1007-9572.2012.11.054
青海地区回族类风湿关节炎患者抗环瓜氨酸抗体与血清肽酰基精氨酸脱亚氨酶基因多态性关系的研究
Anti-cyclic Citrullinated Peptide Antibody and Serum Peptidylarginine Eiminase Polymorphism in Rheumatoid Arthritis Patients in Qinghai Hui Population
摘要
Abstract
Objective To investigate the anti - cyclic citrullinated peptide ( CCP ) antibody levels in patients with rheumatoid arthritis and explore its relationship with serum peptide arginine eiminase polymorphism. Methods The serum anti -CCP antibody level in 63 RA patients and 54 healthy controls was determined with enzyme linked immunosorbent assay ( ELISA ). Erythrocyte sedimentation rate ( ESR ) was measured with Westergren method. C - reactive protein ( CRP ) and rheumatoid factor ( RF ) were determined with immune scatter turbidimetry. RA PADI4 -94, PADI4 - 104 and PADI4 -92 gene polymorphism sites were identified in GenBank ( NT_ 004610 ). The polymorphism types were amplified with polymerase chain reaction and the DNA fragments were sequenced. Results There were significant differences between the two groups compared with ESR, CRP, anti - CCP and RF levels ( P < 0. 05 ). The average disease activity score ( DAS28 ) was ( 4. 89 ± 0. 17 ), and the quality of life score ( HAQ ) was ( 8. 07 ± 1. 03 ). The genotypes in RA PADI4 -94 were A/A, G/G, and A/G, in PADI4 -104 were A/A, G/G, and A/G, and in PADI4 -92 were C/C, G/G, and G/C. The CCP level had no significant differences among these types ( P >0. 05 ). Pearson's correlation analysis showed that the anti - CCP antibody level was positively correlated with DAS28 (r=0. 25, P =0.018), HAQ (r=0. 22, P = 0. 04), and ESR ( r = 0. 81, P =0.001); However, no correlation was observed between the anti - CCP antibody and CRP and RF. Conclusion The anti - CCP antibody level is high among RA patients than the healthy individuals among Hui population and is positively correlated with DAS28 scores, HAQ scores, and ESR. It may be a useful indicator for assessing the disease activity in population. The development of PADI4 polymorphism may be not associated with the anti - CCP antibody.关键词
关节炎,类风湿/肽酰基精氨酸脱亚氨酶/多态性,单核苷酸/抗环瓜氨酸抗体Key words
Arthritis, rheumatoid/ Peptidylarginine eiminase/ Polymorphism, single nucleotide/ Anti -cyclic citrullinated peptides antibodies分类
医药卫生引用本文复制引用
刘冀,杨发满,李蓉,张培莉,汪元浚..青海地区回族类风湿关节炎患者抗环瓜氨酸抗体与血清肽酰基精氨酸脱亚氨酶基因多态性关系的研究[J].中国全科医学,2012,15(32):3734-3736,3.基金项目
2010年青海省科技厅自然基金课题(2010-Z-725) （2010-Z-725）
