中国人兽共患病学报2012，Vol.28Issue(8)：807-810,4.DOI:10.3969/cjz.j.issn.1002-2694.2012.08.010
尖吻蝮蛇舌形虫感染小鼠血清中特异性抗体和循环抗原的动态观察
Dynamics of specific antibody and circulating antigen in serum from the mice infected with Armillifer agkistrodontis
摘要
Abstract
In this study,adults of Armillifer agkistrodontis (A.agkistrodontis) were collected from Agkistrodon acutus,and then the eggs were separated to feed mice.In the next step,when the infection model was established,blood serum of infected mice were collected after 1,2 and 3 weeks,respectively.Furthermore,ELISA and dot- ELISA were used to detect the dynamic change of specific antibodies and circulating antigens respectively.The specific antibodies increased from 8th week,reached the top at 12th week,decreased from 16th week,and then maintain at the same level constantly.Meanwhile,the specific antibodies were typed.It is evident that IgM antibody appeared first.However,it was substitute by IgG1 after 16 weeks.Moreover,the circulating antigens have been detected in the 1st week by dot-ELISA.Then,the dilution between 1:8 to 1:128were founded in 3rd week.The highest dilution with 1:256 appeared at 8th week,maintained before 11th week and then decreased gradually,which might provide a significant clinical implication for early diagnosis of circulating antigens.关键词
尖吻蝮蛇舌形虫/循环抗原/特异性抗体/小鼠Key words
Armillifer agkistrodontis/ circulating antigen/ specific antibody/ mice分类
医药卫生引用本文复制引用
李浩,陈韶红,张永年,郭俭,常正山,陈家旭..尖吻蝮蛇舌形虫感染小鼠血清中特异性抗体和循环抗原的动态观察[J].中国人兽共患病学报,2012,28(8):807-810,4.基金项目
国家科技重大专项(2008ZX10004-001)和(2008ZX10004-011)联合资助 （2008ZX10004-001）
Supported by the National Science and Tcchnology Major Project of China (No.2008ZX10004-001&2008ZX10004-011) （No.2008ZX10004-001&2008ZX10004-011）
