中国人兽共患病学报2012，Vol.28Issue(8)：825-827,836,4.DOI:10.3969/cjz.j.issn.1002-2694.2012.08.014
牛隐孢子虫多重PCR方法的建立及应用
Development and application of multiplex PCR assay for detection of dairy Cryptosporidium
摘要
Abstract
Two pairs of specific primer were designed and synthesized according to the specific sequence of COWP and ITS-1 gene of dairy Cryptosporidium. A multiplex PCR was developed to amplify specific DNA fragment of C. parvum and C. andersoni with the target sequences of 1 033 bp and 263 bp. By optimizing the PCR response condition specific test and the PCR products sequencing, the multi-PCR was established. A total of 1 613 dairy fecal samples isolated from different areas of Guangxi were tested by the multiplex PCR, and then 189 C. andersoni samples and 1 C. parvum sample were detected, which was consistent with conventional Cryptosporidium isolation and identification results. It showed that the established multi-PCR is suitable for the clinical detection and molecular epidemiology survey of dairy Cryptosporidium.关键词
微小隐孢子虫/安氏隐孢子虫/多重PCRKey words
Cryptosporidium parvum / Cryptosporidium andersoni/ multiplex PCR分类
农业科技引用本文复制引用
彭昊,李军,陶立,陈泽祥,韦志锋,许力干,杨威..牛隐孢子虫多重PCR方法的建立及应用[J].中国人兽共患病学报,2012,28(8):825-827,836,4.基金项目
农业部公益性项目资助项目(201103008)、广西水产畜牧兽医局资助项目(桂渔牧财[2011]52号)、广西基本科研业务费专项(桂科专项12-2)联合资助 （201103008）
