中国人兽共患病学报2012，Vol.28Issue(8)：832-836,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.08.016
多重套式RT-PCR检测患者凝血块中登革病毒RNA
Detection of dengue virus RNA in blood clots by multiplex nested reverse transcription-PCR
摘要
Abstract
Dengue is the most common vector borne viral disease of humans globally.Detection of viral RNA from suspected patient specimens is rapid,specific and confirmative in laboratory diagnosis of dengue infections during the acute phase.In this study,a multiplex nested reverse transcription PCR (RT-PCR) system was established for clinical specimens.While other nucleic acid amplification tests showed relatively low sensitivity,the multiplex nested RT PCR assay detected 4 cases among blood clots from 8 serologically confirmed dengue patients.These results suggested that blood clots of dengue patients could be used in laboratory diagnosis,and that the multiplex nested RT PCR assay,which simplified the detection procedure,could facilitate viral RNA detection of specimens in clinical laboratories.关键词
登革病毒/多重套式RT-PCR/核酸检测Key words
dengue virus/ multiplex nested RT-PCR/ nucleic acid amplification test分类
医药卫生引用本文复制引用
张拥军,黄萌,翁育伟,郑友限,王金章..多重套式RT-PCR检测患者凝血块中登革病毒RNA[J].中国人兽共患病学报,2012,28(8):832-836,5.基金项目
福建省自然科学基金项目(2011J01126),福建医学创新课题(2012-CXB-13) （2011J01126）
Supported by the Natural Science Foundation of Fujian Province (No.2011J01126),and the Innovative Projects in Medical Sciences of Fujian Province(No.2012-CXB-13) （No.2011J01126）
