中国人兽共患病学报2012，Vol.28Issue(10)：969-972,4.DOI:10.3969/cjz.j.issn.1002-2694.2012.10.001
结肠弯曲菌Real-time PCR检测方法的建立
Real-time PCR development for identification of Campylobacter coli from stool specimen
摘要
Abstract
The aim of this study is to develop the real-time PCR assays to detect and qualify C. coli from stool specimen. The primers and probe were designed based on the specific sequence of ceuE gene in C. coli using Primer 5.0 and Vector NTI Suite 6.0 e. The PCR assay was optimized with the reference C. coli strains. The standard curve based on the dilutions of genomic DNA showed a linear relationship between log CFU/mL and threshold cycles. The detectable limitation was 5.62 CFU/mL by using purified DNA from bacteria culture. The reproducibility of this assay was assessed by calculating the variation of the threshold cycle value and the slope from test repeats for the same strains and different strains. Our results indicate that the developed assay has high sensitivity and specificity for identification of C. coli.关键词
结肠弯曲菌/实时PCR/ceuEKey words
Campylobacter coli/ real-time PCR/ ceuE分类
医药卫生引用本文复制引用
乔博,许学斌,顾一心,刘国栋,赵飞,何利华,张建中,张茂俊..结肠弯曲菌Real-time PCR检测方法的建立[J].中国人兽共患病学报,2012,28(10):969-972,4.基金项目
国家自然科学基金(No.81071314)Supported by the National Natural Science Foundation(No.81071314) （No.81071314）
