中国人兽共患病学报2012，Vol.28Issue(10)：987-990,4.DOI:10.3969/cjz.j.issn.1002-2694.2012.10.005
登革病毒IgM抗体快速检测试纸条检测效果的初步研究
Preliminary evaluation on rapid detection effect of immuno-chromatography strip for dengue virus IgM antibody
摘要
Abstract
This study conduct the evaluation on performance of dengue IgM antibody immuno chromatography strip (ICS). Two forms of ICS, the general ICS assembled with combined recombinant antigens (rAg) of four serotypes and the typ ing ICS assembled with distinct serotype of rAg, were applied to detect the IgM antibody and type the infection of virus. The result of the general ICS test coincided well with that of IgM antibody capture ELISA (Mac ELISA) test with a 90. 56% overall identity, and the results of this two methods showed no significant difference (P?0. 05). The ICS showed lower cross reactivi ty than Mac ELISA when detecting the IgM antibody against other infection agents. The typing strips showed intertype cross reactivity excepting for the Dl strip. This study implies the potential application of the general ICS in laboratorial diagnosis of dengue virus infection, but its efficacy needs further evaluation with large scale of specimens. And the typing ICS needs further improvement.关键词
登革病毒/IgM/免疫层析/快速检测/血清分型Key words
dengue virus/ immunoglobulin M/ immuno chromatography/ rapid test/ serotyping分类
医药卫生引用本文复制引用
翁育伟,张长弓,王金章,林梅清,黄萌,严延生..登革病毒IgM抗体快速检测试纸条检测效果的初步研究[J].中国人兽共患病学报,2012,28(10):987-990,4.基金项目
福建省科技重点项目(2010Y0010)和“863”计划重点项目(2011AA02A114)联合资助 （2010Y0010）
