中国人兽共患病学报2012，Vol.28Issue(10)：1012-1016,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.10.011
鸡肉生产链中空肠弯曲杆菌的污染分析及PFGE分型研究
Contamination analysis and PFGE typing of Campylobacter jejuni isolated from chicken production chain
摘要
Abstract
This study aims to understand the distribution and spread of Camp ylobacter jejuni in chicken production chain. According to the standards of GB/T4789. 9 2008, SN0175 2010, ISO10272 1:2006, FDA/BMA: 2001 and USDA/ FSIS; 1998, an effective isolation method was developed for detecting suspect Camp ylobacter jejuni contained chicken. The i solates were accurately identified by the duplex PCR based on the sequence of 16S rDNA and hipO gene. A total of 519 samples of the retail raw chicken and feces were examined for the presence of Campylobacter jejuni. From the chicken production chain, 180 Campylobacter jejuni strains were isolated, with an isolation rate of 34. 87%. The higher contamination rates were observed in feces from farms and slaughterhouse (74. 29% and 66. 38%, respectively). Whereas, the contamination rates in chicken from slaughterhouse and market were low (17. 33% and 13. 66% , respectively). The PFGE genotyping of 83 Campy lobacter jejuni isolates yielded 45 PFGE types. This demonstrated that the Campylobacter jejuni were widespread in chicken production chain, and the phenomenon of horizontal transmission of Campylobacter jejuni may existed in chicken production chain.关键词
空肠弯曲杆菌/鸡肉生产链/分离/鉴定/PFGEKey words
Campylobacter jejuni/ chicken production chain/ isolation/ identification/ PFGE分类
医药卫生引用本文复制引用
陈荀,刘书亮,吴聪明,韩新锋,陈栎娜,陆思琴..鸡肉生产链中空肠弯曲杆菌的污染分析及PFGE分型研究[J].中国人兽共患病学报,2012,28(10):1012-1016,5.基金项目
公益性行业(农业)科研专项子课题(200903055)资助 （农业）
