中国人兽共患病学报2012，Vol.28Issue(11)：1065-1070,1101,7.DOI:10.3969/cjz.j.issn.1002-2694.2012.11.002
大肠杆菌O157:H7菌毛hcpA基因产物的小鼠免疫保护试验
Immunoprotection of recombinant hcpA protein from enterohemorrhagic Escherichia coli O157∶H7 in mice
摘要
Abstract
The recently-identified hemorrhagic coli pilus (HCP) gene in E. coli O157∶H7 has been shown to be involved in bacterial adherence, colonization and pathogenesis. In this study, hcpA genes were detected in fifteen O157∶H7 isolates by PCR, and the expression of this type IV pilus was observed by electron microscopy. Meanwhile, the hcpA gene was cloned, and its recombinant HcpA protein was expressed in BL21 (DE3) cells. High-titer polyclonal antibodies (1∶12 800) were prepared with His-tagged HcpA protein in immunized rabbits, thus, the immunogenicity of HCP was analyzed by Western blotting assay. Balb/c mice subcutaneously inoculated with purified His-HcpA protein were challenged by O157∶H7 strains, resulting 70% protection rate and reduced fecal shedding in comparison to naive mice. The observation suggests that the recombinant His-tagged HcpA has considerable immunogenicity in a mouse model, and that HCP is a potential virulent component in E. coli O157∶H7.关键词
肠出血性大肠杆菌O157:H7/Ⅳ型菌毛/表达/排菌Key words
EHEC O157:H7:HCP:immunogenicity:fecal shedding:mouse分类
医药卫生引用本文复制引用
张雪寒,何孔旺,叶青,倪艳秀,温立斌,李彬,周俊明,王小敏..大肠杆菌O157:H7菌毛hcpA基因产物的小鼠免疫保护试验[J].中国人兽共患病学报,2012,28(11):1065-1070,1101,7.基金项目
国家支撑计划(No.2012BAK08B07)和国家自然科学基金(No.31201919)联合资助 （No.2012BAK08B07）
Financially supported by the National Science &Technology Pillar Program (No.2012BAK08B07),and the National Natural Sciences Foundation of China (No.31201919) （No.2012BAK08B07）
