中国人兽共患病学报2012，Vol.28Issue(11)：1102-1106,5.DOI:10.3969/cjz.j.issn.1002-2694.2012.11.009
亚洲带绦虫六钩蚴Ta8基因的克隆表达及免疫原性分析
Cloning, expression and immunogenicity analysis of Ta 8 gene of Taenia asiatica oncospheres
摘要
Abstract
The novel gene named oncosphere 8 kDa gene of Taenia asiatica (Ta8) was cloned and expressed in order to evaluate the value of the reeomhinant protein in the diagnosis . The full-length eDNA of Ta8 gene was cloned from Taeniaasi-atica by using RT-PCR and cloned into the prokaryotic expression vector pET-28a (＋) , and then expressed in E. coli BL21 with IPTG induction . The reeomhinant protein was detected by SDS-PAGE and purified by Ni-IDA affinity chromatography . Its immunogenicity was examined by Western blot . PCR , double enzyme digestion and DNA sequencing confirmed that the re-combinant expression plasmid was successfully constructed . The expression products were obtained and purified by Ni-IDA affinity chromatography . Western blot analysis of Ta8 recombinant protein testified that it could be recognized by immunized SD rat serum while could not be recognized by normal SD rat , thereiore indicated its immunogenicity . A novel gene coding Ta8 of Taenia asiatica was cloned ,expressed ,purified and identified successfully ,which proofing that Ta 8 protein had immunogenicity . Additionally, the Ta 8 gene was also detected in scolex and mature and gravid proglottids of adult Taenia asiatica.关键词
亚洲带绦虫/六钩蚴/基因克隆/原核表达/免疫原性Key words
Taenia asiatica/ oncosphere/ molecular cloning/ prokaryotic expression/ immunogenicity分类
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王宇,程金芝,黄江,戴佳琳,陈小睿,廖兴江..亚洲带绦虫六钩蚴Ta8基因的克隆表达及免疫原性分析[J].中国人兽共患病学报,2012,28(11):1102-1106,5.基金项目
国家自然科学基金 (No.30760227)和贵州省科技攻关项目[黔科合NY字(2008)3060]联合资助 （No.30760227）
