中国人兽共患病学报2012，Vol.28Issue(11)：1120-1125,6.DOI:10.3969/cjz.j.issn.1002-2694.2012.11.013
粤北山区并殖吸虫流行分布现状初步研究
Epidemiological survey of Paragonimus in northern Guangdong Province, China
摘要
Abstract
The objective is to determine the epidemiological distribution of Paragonimus in northern Guangdong Province . Cercariae and metacercariae of Paragonimus were isolated from freshwater snails and crabs , and randomly collected at each study sites in northern Guangdong Province . Adult worms were obtained from experimentally infected cats and dogs . The gene sequences of cytochrome c oxidase subnnit 1 (COI) and second internal transcribed spacer (ITS2) region from adult worms were aligned with those of Paragonimus spp. deposited in GenBank database . Infection rates for the metacercariae of Euparagonimus cenocopiosus in Sinopotamonpinheense recorded at five of the six sites studied were as follows : 74 .54% (41/ 53) at Lidong, 68.91% (32/47) at Soiiliynan, 77.77% (24/32) at Xiadonghe , 76 .92% (40/52) at Daping , and 81 .5% (22/ 27) at Xiaokeng , respectively . The COI gene sequences of adult worms derived from cats and dogs infected with metacercariae from the above five sites shared 99 .5% , l00% , 99 .5% , 99 .5% , and 100% homology with those from Euparagonimus cenocopiosus in GenBank , and the ITS2 gene was l00% identical. At Dadong , however , 38 .09% (32/84) of Sinopotamon pinheense were infected with the metacercariae of Paragonimus westermani , and 0 .058% (1/1 700) of snails (SemisulcospiraLib-ertina) was infected with cercariae of Paragonimus westermani . The COI gene sequence of adult worms from Dadong was 100% identical to that of Paragonimus westermani in the GenBank , and the ITS2 gene sequence was 99 .5% identical. Five new high endemic areas of Euparagonimus cenocopiosus , with Sinopotamon pinheense as second intermediate host, and one new high endemic area of Paragonimus westermani , utilizing Semisulcospira Libertina and Sinopotamon pinheense as first and second intermediate hosts , were dis- red in northern Guangdong .关键词
并殖吸虫/尾蚴/囊蚴/感染率/放逸短沟蜷/平和华溪蟹Key words
Paragonimus / cercariae / metacercariae / infection rate / Semisulcospira liber tine / Sinopotamon pinheense分类
医药卫生引用本文复制引用
傅广华,邓文强,刘巧,陆予云,李旭文,陈少华,李宏光,王萌..粤北山区并殖吸虫流行分布现状初步研究[J].中国人兽共患病学报,2012,28(11):1120-1125,6.基金项目
广东省医学科研基金项目(No.A2009248 （）
No.A 2010638) （）
广州市教育局科研基金项目(No.10 A198) （No.10 A198）
韶关市科技计划项目(No.201038) （No.201038）
广州医学院卫生职业技术学院重点项目(No.2012005).联合资助 （No.2012005）
