中国水稻科学2012，Vol.26Issue(6)：651-655,5.DOI:10.3969/j.issn.1001-7216.2012.06.003
细胞膜质子泵基因超表达促进水稻种子萌发
Overexpression of a Plasma Membrane H+-ATPase Gene Promotes Rice Seed Germination
摘要
Abstract
To verify the direct effect of H+-ATPase on the root cell elongation, the T2 generation seeds of the rice plasma membrane H+-ATPase gene OsA8 (AJ440219) overexpressed transgenic materials ( OE1) were used as samples. Firstly, the differences in seed germination rate and the growth rate of primary roots between the overexpressed line and the wild-type were determined. Secondly, the activities of H+ -ATPase in the root cell membranes isolated by two-phase separation method were compared and then the OsA8 gene expression at the transcriptional level was determined. The protein concentration of H+-ATPase of these two materials was analyzed by Western blot. The results showed that not only the germination rate but also the growth rate of primary roots of OE1 were higher than that of wild-type. In addition, OE1 has much higher activity of the root plasma membrane H+-ATPase than wild-type. The expression of OsA8 gene and protein concentration of OE1 was also higher than that of wild-type. The results indicated that the overexpression of the plasma membrane H+-ATPase gene OsA8 in the OE1 rice is the main factor of the elevation of the H + -ATPase activity, which contributed to the increased seed germination rate and growth of primary root.关键词
水稻/细胞膜H+-ATPase/OsA8/萌发Key words
rice/ plasma membrane H+ -ATPase/ OsA8 / germination分类
生物科学引用本文复制引用
刘赣,张明超,曾后清,朱毅勇,孙淑斌,徐国华..细胞膜质子泵基因超表达促进水稻种子萌发[J].中国水稻科学,2012,26(6):651-655,5.基金项目
国家自然科学基金资助项目(30971864) （30971864）
教育部新世纪优秀人才资助项目(NCET-11-0672). （NCET-11-0672）
