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基于SSILP、InDel和SSR标记的杂草稻籼粳分类

徐群 许红云 魏兴华 汤圣祥 李东宣 陈丽娟

中国水稻科学2012,Vol.26Issue(6):686-692,7.
中国水稻科学2012,Vol.26Issue(6):686-692,7.DOI:10.3969/j.issn.1001-7216.2012.06.008

基于SSILP、InDel和SSR标记的杂草稻籼粳分类

SSILP, InDel and SSR Markers-based indica-japonica Classification for Weedy Rice

徐群 1许红云 2魏兴华 1汤圣祥 1李东宣 2陈丽娟2

作者信息

  • 1. 中国水稻研究所水稻生物学国家重点实验室,浙江杭州310006
  • 2. 云南农业大学稻作研究所,云南昆明650201
  • 折叠

摘要

Abstract

Forty SSILP, 30 InDel and 43 SSR markers were used to compare genetic diversity of 42 weedy and cultivated rice entries from Sri Lanka, 9 indica and 7 japonica rice cultivars as well as four representatives of weedy rice from China and other countries (regions). There were 84, 61 and 213 alleles identified by SSILP, InDel and SSR markers, with average polymorphism information content (PIC) values 0. 257, 0. 255 and 0. 543, respectively. The N-J clusters analysis indicated all 42 Sri Lanka weedy and cultivated rice entries belonged to indica type. The genetic distances of SSILP and InDel markers revealed highly correlation (r=0. 989). Meanwhile the associations between SSR markers and the other two markers (SSILP and InDel) were lower as 0. 812 and 0. 808, respectively. With the rising reliability and accuracy of indica-japonica classification resulted from the development of more subspecies-specific markers, SSILP and InDel markers can be applied extensively for the indica-japonica classification of rice germplasm. And SSR markers can classify better in the subspecies.

关键词

杂草稻/栽培稻/分子标记/籼粳分类

Key words

weedy rice/ cultivated rice/ molecular marker/ indica-japonica classification

分类

生物科学

引用本文复制引用

徐群,许红云,魏兴华,汤圣祥,李东宣,陈丽娟..基于SSILP、InDel和SSR标记的杂草稻籼粳分类[J].中国水稻科学,2012,26(6):686-692,7.

基金项目

国家自然科学基金资助项目(30860057) (30860057)

国家973计划资助项目(2011CB100401). (2011CB100401)

中国水稻科学

OA北大核心CSCDCSTPCD

1001-7216

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