中国神经再生研究（英文版）2012，Vol.7Issue(31)：2432-2438,7.DOI:10.3969/j.issn.1673-5374.2012.31.004
Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion
Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion
Fengzhang Wang 1Yuan Zhang 2Chunke He 3Tingting Wang 4Qiyan Piao 5Qun Liu1
作者信息
- 1. Department of Neurology, Bethune First Hospital, Jilin University, Changchun 130021, Jilin Province, China
- 2. Department of Neonatology, Bethune First Hospital, Jilin University, Changchun 130021, Jilin Province, China
- 3. Department of Orthopedics, Jilin Hospital of Integrated Traditional and Western Medicine, Changchun 130021, Jilin Province, China
- 4. Department of Infectious Diseases, Zibo First Hospital, Zibo 255200, Shandong Province, China
- 5. Department of Cardiology, General Hospital of China National Petroleum Corporation in Jilin, Jilin 132021, Jilin Province, China
- 折叠
摘要
Abstract
C/EBP homologous protein, an important transcription factor during endoplasmic reticulum stress, participates in cell apoptosis mediated by endoplasmic reticulum stress. Previous studies have shown that C/EBP homologous protein mediates nerve injury during Alzheimer's disease, subarachnoid hemorrhage and spinal cord trauma. In this study, we introduced C/EBP homologous protein short hairpin RNA into the brains of ischemia/reperfusion rat models via injection of lentiviral vector through the left lateral ventricle. Silencing C/EBP homologous protein gene expression significantly reduced cerebral infarction volume, decreased water content and tumor necrosis factor-α and interleukin-1β mRNA expression in brain tissues following infarction, diminished the number of TUNEL-positive cells in the infarct region, decreased caspase-3 protein content and increased Bcl-2 protein content. These results suggest that silencing C/EBP homologous protein lessens cell apoptosis and inflammatory reactions, thereby protecting nerves.关键词
C/EBP homologous protein/endoplasmic reticulum stress/Alzheimer's disease/subarachnoid hemorrhage/tumor necrosis factor-α/ischemia/reperfusion/interleukin-1β/cerebral infarction/neural regenerationKey words
C/EBP homologous protein/endoplasmic reticulum stress/Alzheimer's disease/subarachnoid hemorrhage/tumor necrosis factor-α/ischemia/reperfusion/interleukin-1β/cerebral infarction/neural regeneration引用本文复制引用
Fengzhang Wang,Yuan Zhang,Chunke He,Tingting Wang,Qiyan Piao,Qun Liu..Silencing the gene encoding C/EBP homologous protein lessens acute brain injury following ischemia/reperfusion[J].中国神经再生研究（英文版）,2012,7(31):2432-2438,7.
