首页|期刊导航|中国神经再生研究（英文版）|Expression of gamma-aminobutyric acid type A receptor α2 subunit in the dorsal root ganglion of rats with sciatic nerve injury
中国神经再生研究（英文版）2012，Vol.7Issue(32)：2492-2499,8.DOI:10.3969/j.issn.1673-5374.2012.32.002
Expression of gamma-aminobutyric acid type A receptor α2 subunit in the dorsal root ganglion of rats with sciatic nerve injury
Expression of gamma-aminobutyric acid type A receptor α2 subunit in the dorsal root ganglion of rats with sciatic nerve injury
摘要
Abstract
The γ-aminobutyric acid neurotransmitter in the spinal cord dorsal horn plays an important role in pain modulation through primary afferent-mediated presynaptic inhibition. The weakening of γ-aminobutyric acid-mediated presynaptic inhibition may be an important cause of neuropathic pain. Γ-aminobutyric acid-mediated presynaptic inhibition is related to the current strength of γ-aminobutyric acid A receptor activation. In view of this, the whole-cell patch-clamp technique was used here to record the change in muscimol activated current of dorsal root ganglion neurons in a chronic constriction injury model. Results found that damage in rat dorsal root ganglion neurons following application of muscimol caused concentration-dependent activation of current, and compared with the sham group, its current strength and γ-aminobutyric acid A receptor protein expression decreased. Immunofluorescence revealed that γ-aminobutyric acid type A receptor α2 subunit protein expression decreased and was most obvious at 12 and 15 days after modeling. Our experimental findings confirmed that the γ-aminobutyric acid type A receptor α2 subunit in the chronic constriction injury model rat dorsal root ganglion was downregulated, which may be one of the reasons for the reduction of injury in dorsal root ganglion neurons following muscimol-activated currents.关键词
γ-aminobutyric acid/γ-aminobutyric acid type A receptor α2 subunit/neuropathic pain/dorsal root ganglion/whole-cell patch clamp/immunofluorescence/primary afferent depolarization/paw withdrawal latency/muscimolKey words
γ-aminobutyric acid/γ-aminobutyric acid type A receptor α2 subunit/neuropathic pain/dorsal root ganglion/whole-cell patch clamp/immunofluorescence/primary afferent depolarization/paw withdrawal latency/muscimol引用本文复制引用
Yu Lian,Yang Wang,Ketao Ma,Lei Zhao,Zhongshuang Zhang,Yuanyuan Shang,Junqiang Si,Li Li..Expression of gamma-aminobutyric acid type A receptor α2 subunit in the dorsal root ganglion of rats with sciatic nerve injury[J].中国神经再生研究（英文版）,2012,7(32):2492-2499,8.基金项目
This study was supported by the Youth Science and Technology Innovation Special Foundation of Xinjiang Production and Construction Corps,China,No.2010JC33. （）
