中国实验动物学报2012，Vol.20Issue(5)：25-28,4.DOI:10.3969/j.issn.1005-4847.2011.05.006
灰仓鼠多房棘球蚴腹腔动物模型的建立
Establishment of an animal model of peritoneal Echinococcus muhilocularis infection in Cricetulus migratorius
摘要
Abstract
Objective To establish an animal model of Echinococcus muhilocularis (E. m) infection in Cricetulus migratorius, and observe the growth of E. m in the peritoneal cavity of C. migratorius. Methods C. migratorius was inoculated into the peritoneal cavity in a dose of 2000 cephalomeres of E. m in each animal. The cyst weight and positive antibody were measured, and the cyst weight/body weight ratio was calculated. The growth of E. m was observed in the C. migratorius at 10, 15, 18, 22, 40, 60 , 80, 100 days after inoculation. Growth and the infection rate of E. m cysts was compared at 100 days after inoculation into 10 animals at a dose of 100, 500 or 2000 E. m cephalomeres, respectively. Results Fifty nine of the 62 C. migratorus were infected by E. m. Mature E. m cephalomeres were observed at 18 days after inoculation and the infection rate reached 100% . Positive antibody was observed at 15 days after inoculation. The cyst weight/body weight ratio was over 15% at 60 days after inoculation. A 100% infection rate was observed at 100 days after inoculation at the three doses. Conclusions An animal model of peritoneal Echinococcus muhilocularis infection in Cricetulus migratorius is successfully established, with the characteristics of high infection rate, short period of infection , easy observation of the growth of E. m and antibody titer on these animal models. Sixty days after inoculation of E. m can be used as observation period of the animal modeling in C. migratorius. The cyst weight/body weight ratio, antibody titer and level of cyst growth can be used as the major reference indicators of E. m infection modeling. Females are better than males for making animal models of E. m infection in C. migratorius.关键词
多房棘球蚴/灰仓鼠/包囊系数/动物模型/抗体/感染Key words
Echinococcus muhilocularis (E. m)/Cricetus migratorius/Cystic index/Animal model/Antibody/Infection分类
生物科学引用本文复制引用
刘斌,徐艺玫,燕顺生,史深,麦丽开,廖力夫..灰仓鼠多房棘球蚴腹腔动物模型的建立[J].中国实验动物学报,2012,20(5):25-28,4.基金项目
国家"十一五"科技支撑计划重点项目(No.2009BAI83B02). （No.2009BAI83B02）
