中国实验动物学报2012，Vol.20Issue(5)：35-40,6.DOI:10.3969/j.issn.1005-4847.2011.05.008
五指山小型猪高脂血症模型的心脏自主神经功能变化
Changes of the autonomic nervous function in Wuzhishan minipig models of hyperlipidemia
摘要
Abstract
Objective The aim of this study was to observe the changes of autonomic nervous function in Wuzhishan minipigs ( WZS) and investigate the relationship between dyslipidemia and 24 h or diurnal changes of autonomic nervous function in these animals. Methods The WZS minipig models of hyperlipidemia was established by feeding with high fat diet. The serum TG, TC, HDL-C and LDL-C levels were detected and electrocardiogram was monitored by non-invasion telemetry every 2 weeks. 24 h or diurnal autonomic nervous function was evaluated by heart rate variability (HRV) analysis. Results The serum TC, HDL-C, LDL-C levels were significantly increased in the WZS minipigs after feeding with high fat diet for 2 weeks ( P < 0. 01) , and TG level was also significantly increased after 6 weeks (P < 0. 05 ). Among them, TC and LDL-C levels were rapidly increased during 0 to 4 weeks and reached a high peak at 4 to 6 weeks, and then maintained stable at a certain level during 8 tolO weeks. HDL-C was also rapidly increased and reached a peak during 0 to 2 weeks, and then maintained stable during 2 to 10 weeks. Meanwhile, HRV time-domain analysis indexes ( including RRI, SDNN, RMSSD, SDANN, PNN50, SDANN index, STV, LTV) and HRV frequency-domain analysis indexes (including TP, VLF, LF, HF) were gradually reduced (P<0. 05, P<0. 01), while LF/HF was significantly increased (P < 0. 05). The degrees of changes of the HRV parameters were slightly higher in the nighttime than those of daytime. The results of linear correlation analysis showed that there were significant correlations between TC, LDL-C, TC/HDL-C, LDL-C/HDL-C and HRV parameters (P<0. 05, P<0. 01). Moreover, the multivariate linear gradual regressive analysis also showed that TC, LDL-C, TC/HDL-C, LDL-C/HDL-C were related with SDNN, and their correlation coefficients were 0. 673, 0. 672, 0. 665 and 0. 681 , respectively. Conclusions Hyperlipidemia induced in WZS minipigs by high fat diet may reduce and disturb the autonomic nervous function. The degree of disturbance of autonomic nervous function may be related with duration of hyperlipidemia status, and the risk of dyslipidemia caused by cardiovascular disease is higher in the nighttime than that in daytime. There is a certain dependence between reduced HRV and increased TC, LDL-C, TC/HDL-C and LDL-C/HDL-C ratio. The reduced SDNN can be considered as a predictive indicator of hyperlipidemia independently associated with the risk of development of cardiovascular disease.关键词
五指山小型猪/高脂血症/心率变异/自主神经功能/动物模型Key words
Autonomic nervous function/Hyperlipidemia/Wuzhishan minipig/Heart rate variability/Animal model分类
生物科学引用本文复制引用
陈亮,潘永明,徐孝平,何欢,朱科燕,杨玉伟,陈民利..五指山小型猪高脂血症模型的心脏自主神经功能变化[J].中国实验动物学报,2012,20(5):35-40,6.基金项目
卫生部科研基金(WKJ2009-2-032) （WKJ2009-2-032）
浙江省卫生高层次创新人才培养工程项目资助. （）
