中国比较医学杂志2012，Vol.22Issue(10)：1-5,5.DOI:10.3969.j.issn.1671.7856.2012.010.001
人载脂蛋白C3基因转基因小鼠的建立及血脂变化分析
Establishment of a Human APOC3 Transgenic Mouse Model and Analysis of the Blood Lipids
摘要
Abstract
Objective To generate transgenic mice expressing human AP0C3, and establish a mouse model of hyperlipidemia. Methods The transgenic plasmid was constructed by inserting human AP0C3 gene into the downstream of the human ubiquitin C ( Ubc) promoter. The transgenic mice were produced by microinjection and the genotyping was detected by PCR. The expression level of the gene was determined by Western blotting. The levels of blood lipids of the mice at different ages were detected using a biochemical analyzer. Fat in the liver tissue was observed by histology with fat staining. Results The transgenic mouse model with overexpression of human AP0C3 gene was established, showing overexpression of human AP0C3 gene in the blood, liver, intestine, muscle, heart, kidney and spleen tissues, comparedwith those of the wild type mice. The plasma triglyeeride phosphate (TG) level and liver fat eontent of the transgenie mice were signifieantly higher than those in the wild type miee. Conclusions A transgenie mouse model overexpressing human AP0C3 gene and phenotypes of hyperlipidemia has been developed, providing a useful animal model for hyperlipidemia and related angioeardiopathy studies.关键词
载脂蛋白C3/转基因小鼠/甘油三酯/高脂血症/人APOC3基因Key words
Apolipoprotein C3/ Transgenie miee/ Triglyeeride/ Hyperlipidemia/ Human AP0C3 gene分类
医药卫生引用本文复制引用
全雄志,高翔,张旭,葛文萍,关菲菲,董伟,张连峰..人载脂蛋白C3基因转基因小鼠的建立及血脂变化分析[J].中国比较医学杂志,2012,22(10):1-5,5.基金项目
国家"重大新药创制"科技重大专项课题"啮齿类研发平台创新药物研究开发技术平台建设"(课题编号2011ZX09307-302). （课题编号2011ZX09307-302）
