中国比较医学杂志2012，Vol.22Issue(10)：6-10,5.DOI:10.3969.j.issn.1671.7856.2012.010.002
抗人B淋巴细胞单克隆抗体清除中国恒河猴体内B淋巴细胞效果观察
B-Lymphocyte Depletion in Chinese Rhesus Macaques with Anti-Human B-Lymphocyte Monoclonal Antibody Rituximab
摘要
Abstract
Objective To observe the effects of rituximab injection, an anti-human B-lymphocyte monoclonal antibody, on eliminating the B lymphocytes from Chinese origin rhesus macaques , and to provide some experimental data for establishing B-cell-depleted animals models and better define the role of B cells in the Control of pathogenic simian immunodeficiency virus (SIV) replication in future studies. Methods Two healthy Chinese origin rhesus monkeys were selected and infused with rituximab via the tail vein by intravenous drip. Peripheral blood, lymph nodes and duodenum biopsy samples were collected at different time points after injection. Lymphocytes were isolated from all the samples , stained and analyzed by FACS. Results The B lymphocytes in peripheral blood of rhesus monkeys were completely depleted 24 hours after the rituiimab infusion and lasted for 14 days, while 100％ depletion in the inguinal lymph nodes and 90％ depletion in the duodenal mueosa at 7 days after infusion. At the same time, CD4+ T and CD8+ T-eells in peripheral blood maintained at a stable level without significant fluctuation. Conclusions B lymphocytes can be effectively eliminated from various tissues in Chinese origin rhesus macaques by rituiimab injection in vivo.关键词
中国恒河猴/B淋巴细胞/利妥昔单抗Key words
Chinese origin rhesus macaques/ B lymphocytes/ Rituiimab分类
医药卫生引用本文复制引用
苏爱华,丛喆,王卫,陈霆,魏强..抗人B淋巴细胞单克隆抗体清除中国恒河猴体内B淋巴细胞效果观察[J].中国比较医学杂志,2012,22(10):6-10,5.基金项目
国家科技重大专项课题(2012ZX10004-501,2012ZX10001-006-003). （2012ZX10004-501,2012ZX10001-006-003）
