中国比较医学杂志2012，Vol.22Issue(10)：33-36,后插1,5.DOI:10.3969.j.issn.1671.7856.2012.010.008
热量限制对SH-SY5Y细胞氧化损伤的影响
Effect of Caloric Restriction on Oxidative Damage in Human Neuroblastoma Cell Line SH-SY5Y Cells
摘要
Abstract
Objective To observe the effect of caloric restriction on oxidative damage in human neuroblastoma cell line SH-SY5Y cells in vitro. Methods To establish an in vitro model of H2O2-induced oiidative stress damage of SH-SY5Y cells. SH-SY5Y cells were cultured in vitro. The cells were divided into four groups: control group, H2O2 (250μmol/L) group, low glucose (2g/L) group, and low glucose + H2 O2 group. Cell morphology, thiazolyl blue (MTT) metabolism rate, and lactate dehydrogenase (LDH) leakage rate were measured to observe the cell growth status in different groups. Results Compared with the control group, the MTT metabolism rate in the cells treated with 50 μmol/L H2O2 for one hour was not significantly changed (P >0. 05) , but it was significantly decreased in the 100, 250, 500, 1000 μmol/ L H202-treated groups than that of control group (P <0. 01) , and there was a tendency that along with the increase of H2 02Concentration, the cell activity was increasingly decreased. So, the H2 02 concentration of 250 μmol/L was selected to generate oiidative stress. The MTT metabolism rate in the cells pretreated with low glucose for 24 hours and treated with 250 μmol/L H2 02 for one hour was significantly decreased than that of control group ( P < 0. 01 ) , but significantly increased than that of the H202group (P<0. 01). When the cells were further cultured for 7 hours, the metabolism rate of the low glucose group was significantly higher than that of the control group (P<0. 01). Compared with the H202group, the metabolism rate of the low glucose + H202group was significantly increased (P<0. 01). The LDH leakage rate in the cells treated with 250 μmol/L H202for 1 hour was significantly increased than that of the control group (P<0. 05). The LDH leakage rate in the low glucose treated group was slightly decreased, and the rate of the low glucose + H202group was significantly lower than that of the H202-treated group (P<0.01). When the cells were further culture for 7 hours, the LDH leakage rate of the low glucose group treated for 7 hours was slightly higher than that of cells cultured for 1 hour (P > 0. 05) . The LDH leakage rate of the low glucose + H202group cultured for 7 hours was slightly higher than that of cells cultured for 1 hour (P >0. 05) . The histological observation revealed that the morphology of cells treated with low glucose was similar to that of the control group, and it was similar at one hour after H202 added. At 7 hours after addition of H202, the cells of the low glucose group and control group had well streching cytoplasmic projections, but in the H202 group, the cell number was significantly reduced, with a lot of dead cells, and the cells became rounded in shape and with poor adherence and transparence. Conclusion Caloric restriction can improve the viability and anti-oxidative stress ability of neurons, and reduce the cell mortality.关键词
氧化应激/SH-SY5Y细胞/细胞培养/热量限制Key words
Oiidative stress/ Human neuroblastoma/ cell line SH-SY5Y/ Cell culture/ Caloric restriction分类
医药卫生引用本文复制引用
陈娟,张景燕,王蓉,赵静姝,郭瑾,吴燕川,赵志炜..热量限制对SH-SY5Y细胞氧化损伤的影响[J].中国比较医学杂志,2012,22(10):33-36,后插1,5.基金项目
北京市自然科学基金(7082043) （7082043）
首都卫生发展科研专项项目(2011-1001-02) （2011-1001-02）
北京市卫生系统高层次卫生技术人才计划资助项目(2009-3-64). （2009-3-64）
