中国比较医学杂志2012，Vol.22Issue(11)：41-45,5.DOI:10.3969.j.issn.1671.7856.2012.011.009
小鼠仙台病毒ELISA抗体检测规范化试剂盒的研制与应用
The Development and Application of Standardized ELISA Kit for Mouse Sendai Virus
摘要
Abstract
Objective To develop a standardized ELISA kit for mouse Sendai virus and test it in clinical application. Methods Sendai virus seed batches and BHK-21 cell banks were set firstly; then the crafts for virus production and antigen purification were established ; the detection system in ELISA flat was also optimized ; the positive and negative serum controls were normalized. A totally 672 sera samples were analyzed by the current ELISA kit , and the results were confirmed by IFA and Western blot. Results The Sendai virus seed batches stored at -80℃ were stable for more than 6 months; the virus production craft and antigen purification craft improved the antigen quality ; the control systems reduced the effects from environment. The detection sensitivity of ELISA kit is higher than Western blot , much higher than IFA. Conclusion The standardized mouse Sendai virus detection ELISA kit can detect the Sendai virus infection condition, and the detection result is stable and reproducible .关键词
小鼠/仙台病毒/ELISA/规范化Key words
Mouse/Sendai virus/ELISA/Standardized分类
医药卫生引用本文复制引用
向志光,佟巍,刘先菊,张丽芳,李雨函,王艳蓉,刘云波,魏强..小鼠仙台病毒ELISA抗体检测规范化试剂盒的研制与应用[J].中国比较医学杂志,2012,22(11):41-45,5.基金项目
中央级公益性科研院所基本科研业务费专项基金项目(DWS201213). （DWS201213）
