中国比较医学杂志2012，Vol.22Issue(11)：50-53,4.DOI:10.3969.j.issn.1671.7856.2012.011.011
鼠尾胶原蛋白提取、分离、纯化方法的建立及鉴定
The Establishment and Appraisal of the Methods for the Extraction, Separation and Purification of Rat Tail Collagen
摘要
Abstract
Objective To establish a highly efficient method for the extraction , separation and purification of rat tail collagen. Methods The rat tail tendon has been obtained through stripping the rat tail ; the rat tail collagen stoste has been developed through processing with Tris -HC1 buffer, pepsin; sodium chloride solution has been repeatedly used for graded salting-out; acetic acid has been used for the purification of collagen . To get purified rat tail collagen , inorganic salt has to be removed with ultra-pure water dialysis. Appraisal is to be made through such technical means such as SDS -PAGE protein electrophoresis, amino acid content analysis. Results Rat tail collagen of high purity can be developed with the method established in this study , whose purity can reach electrophoresis level . When compared with those commercialized rat tail collagen products imported from overseas there is no difference . The effects the parameters for the extraction , separation and purification have exerted on yield and purity has been deliberated , established The optimal conditions for the extraction of rat tail collagen has been established, pepsin usage; l:500, enzyme solution time : 72 h, salting-out concentration; 2 mol/L, the acid solution used for extraction : 0.05 mol/L acetum. Conclusion For rat tail collagen enlargement production to provide the proper processing parameters have been provided for the enlargement of production of rat tail collagen, for a large gain rat tail collagen and deeper efficacy research provides the theoretical support and practical basis have also been provided for the obtaining of large quantities of rat tail collagen so as to carry out deeper research on its efficacy.关键词
鼠尾胶原/蛋白质提取/蛋白质分离/蛋白质纯化/蛋白质鉴定Key words
Rat tail collagen/Protein extracted/Protein separation/Protein purification/Protein appraisal分类
医药卫生引用本文复制引用
任海涛,钟志勇,郑佳琳,饶子亮,邝少松,王刚,唐小江..鼠尾胶原蛋白提取、分离、纯化方法的建立及鉴定[J].中国比较医学杂志,2012,22(11):50-53,4.基金项目
广东省医学实验动物中心科研项目(2012001). （2012001）
