中国兽医科学2012，Vol.42Issue(11)：1121-1127,7.
成年大熊猫肠道菌群多样性的16S rDNA-RFLP分析
Research on diversity of intestinal flora in an adult giant panda based on the 16 S rDNA-RFLP
摘要
Abstract
To investigate the diversity of intestinal bacteria of giant pandas in Autumn(September to November), the 16 S rDNA of total bacteria was amplified with bacterial universal primer of 16 S rDNA and the 16 S rDNA clone libraries were constructed. The diversity of 16 S rDNA clone library was analyzed by 16 S rDNA-restriction fragment length polymorphism(RFLP) technique. Results showed that the dominant microflora in giant panda's intestine were Escherichia coli, Pseudomonas, Streptococcus and Sphingobacterium. The diversity of bacteria in the intestinal tract of giant panda in September was higher than that in November. Results demonstrated that the technology based on 16 S rDNA-RFLP can be used to the study on diversity of intestinal flora in giant panda.关键词
大熊猫/肠道菌群/多样性/16S/rDNA/限制性片段长度多态性Key words
giant panda/intestinal flora/diversity/16 S rDNA/RFLP分类
农业科技引用本文复制引用
何廷美,张婷婷,罗永久,程安春,彭广能,崔婷婷,钟志军,王承东,何永果,王燚,张和民,李德生,李才武..成年大熊猫肠道菌群多样性的16S rDNA-RFLP分析[J].中国兽医科学,2012,42(11):1121-1127,7.基金项目
国家自然科学基金资助项目 （）
大熊猫国际合作资金项目 （）
四川省2011年学术和技术带头人培养资金资助项目 （）
教育部“长江学者和创新团队发展计划”创新团队项目 （）
四川省科技厅科技支撑项目 （）
四川农业大学双支计划项目 （）
